Uterine volume and endometrial thickness in healthy girls evaluated by ultrasound (3- dimensional) and magnetic resonance imaging  Casper P. Hagen, M.D., Ph.D., Annette Mouritsen, Ph.D., Mikkel G. Mieritz, M.D., Jeanette Tinggaard, M.D., Christine Wohlfahrt-Veje, Ph.D., Eva Fallentin, M.D., Vibeke Brocks, M.D., Karin Sundberg, D.M.Sc., Lisa Neerup Jensen, M.D., Anders Juul, D.M.Sc., Katharina M. Main, Ph.D.  Fertility and Sterility  Volume 104, Issue 2, Pages 452-459.e2 (August 2015) DOI: 10.1016/j.fertnstert.2015.04.042 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Uterus of a prepubertal girl (Tanner stage B1), age 11.3 years (left), and Tanner stage B4, age 12.1 years (right), visualized by (A and B) TAUS; (C and D) a 3D TAUS model of uterine volume; and (E and F) MRI. Fertility and Sterility 2015 104, 452-459.e2DOI: (10.1016/j.fertnstert.2015.04.042) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Uterine volume: (A) ellipsoid TAUS; (B) 3D TAUS; (C) ellipsoid MRI, according to pubertal breast stages (B1–B5). Endometrial thickness: (D) TAUS; and (E) MRI, according to pubertal breast stages. Boxplots indicate median (fat black line), 25th–75th percentile (box), and 2.5th–97.5th percentile (hinge). Fertility and Sterility 2015 104, 452-459.e2DOI: (10.1016/j.fertnstert.2015.04.042) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Correlations (left) and Bland-Altman plots (right) comparing methods evaluating uterine volume and endometrial thickness. (A and B) Ellipsoid TAUS volume vs. 3D TAUS volume; (C and D) ellipsoid TAUS volume vs. ellipsoid MRI volume; and (E and F) endometrium TAUS vs. endometrium MRI. Tendency lines for correlations are shown in black; reference lines are shown in red. Tanner stages are shown by color: B1 (green); B2 (orange); B3 (gray); B4 and 5 (red); unknown (white). Fertility and Sterility 2015 104, 452-459.e2DOI: (10.1016/j.fertnstert.2015.04.042) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Results of multiple regression analyses evaluating which follicles contributed most to uterine volume and endometrial thickness. The y-axis indicates follicle numbers stratified by diameters (mm), assessed using MRI and TAUS. The estimated effect of a 2-fold increase (doubling) in follicle numbers is presented as a geometric mean (95% CI). Both TAUS (red) and MRI (gray) revealed that uterine volume (A) and endometrial thickness (B) are affected by the number of large, but not small, follicles. Fertility and Sterility 2015 104, 452-459.e2DOI: (10.1016/j.fertnstert.2015.04.042) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Correlations (left) and Bland-Altman plots (right) comparing TAUS and MRI evaluation of: (A and B) uterine length; (C and D) width; and (E and F) depth. Tendency lines for correlations are black; reference lines are red. Tanner stages are shown by color: B1 (green); B2 (orange); B3 (gray); B4 and 5 (red); unknown (white). Fertility and Sterility 2015 104, 452-459.e2DOI: (10.1016/j.fertnstert.2015.04.042) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions