Christine Formisano-Tréziny, Marina de San Feliciano, Jean Gabert

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Presentation transcript:

Development of Plasmid Calibrators for Absolute Quantification of miRNAs by Using Real-Time qPCR Christine Formisano-Tréziny, Marina de San Feliciano, Jean Gabert The Journal of Molecular Diagnostics Volume 14, Issue 4, Pages 314-321 (July 2012) DOI: 10.1016/j.jmoldx.2012.02.008 Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 qPCR amplification of two independent hsa-let-7b plasmid calibrator ranges (A and B). Amplification plot (left panel) of 5 points (102, 103, 104, 105, and 106 copies) measured in duplicate. Calibration curve (right panel) with equation and R2 of the linear regression are indicated. The Journal of Molecular Diagnostics 2012 14, 314-321DOI: (10.1016/j.jmoldx.2012.02.008) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Classical (thin line) and alternative (thick line) strategy workflow. The Journal of Molecular Diagnostics 2012 14, 314-321DOI: (10.1016/j.jmoldx.2012.02.008) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions