Neutrophil antifungal response in CCR2-depleted mice is rescued by adoptive transfer of CCR2+ monocytes or by treatment with recombinant IFNs. Neutrophil.

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Neutrophil antifungal response in CCR2-depleted mice is rescued by adoptive transfer of CCR2+ monocytes or by treatment with recombinant IFNs. Neutrophil antifungal response in CCR2-depleted mice is rescued by adoptive transfer of CCR2+ monocytes or by treatment with recombinant IFNs. CD45.2+CCR2-depleted mice were infected with Af and either left untreated or treated by the adoptive transfer of CD45.1+CCR2-GFP+ monocytes at 3 hours after infection. Monocytes (~850,000) were isolated from the lung of Af-infected CD45.1+CCR2-GFP+ donor mice 3 hours after infection. (A) Representative FACS plot of control, CCR2-depleted mice, and CCR2-depleted mice that received CD45.1+CCR2+ monocytes. (B and C) WT control (black symbols), CCR2-depleted (red symbols), or CCR2-depleted mice adoptively transferred with CCR2+ monocytes (blue symbols) were examined at 48 hours after infection for ROS generation by neutrophils (B) or Af fungal burden (C) in the lung. Each symbol represents one mouse. Data shown are cumulative of two independent experiments. (D and E) CCR2-depleted mice were left untreated or treated with recombinant IFN-α2 and/or IFN-λ3 as indicated. Mice were treated with 1 μg of individual cytokines, except for the double-treated, which received 500 ng of IFN-α2 and 500 ng of IFN-λ3. To recapitulate the kinetics of IFN expression in control mice, we treated CCR2-depleted mice shortly after infection with type I IFN followed by type III IFN. Mice were treated with IFNs the same day of infection and then every other day. (D) Analysis of ROS generation by airway-infiltrating neutrophils isolated from controls (WT littermates treated with DT) or from untreated and treated CCR2-depleted mice. (E) Pulmonary fungal burden 48 hours after infection. Each symbol represents one mouse. Data shown are for one experiment representative of two independent ones. (F) Kaplan-Meier survival plot of CCR2-depleted mice treated with each IFN and mock-treated controls. (G) Representative images of Gomori ammoniacal silver–stained lung sections captured at ×40 magnification. Data shown are cumulative of two to three independent experiments. *P < 0.05; ***P < 0.001; ****P < 0.0001 for each labeled sample as compared with CCR2-depleted mice, calculated by Mann-Whitney or Kruskal-Wallis nonparametric statistical tests using Prism software. Vanessa Espinosa et al. Sci. Immunol. 2017;2:eaan5357 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works