Volume 124, Issue 3, Pages (March 2003)

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Volume 124, Issue 3, Pages 701-707 (March 2003) Liver regeneration in heparin-binding EGF-like growth factor transgenic mice after partial hepatectomy Shinichi Kiso, Sumio Kawata, Shinji Tamura, Yoshiaki Inui, Yuichi Yoshida, Yoshiyuki Sawai, Shinobu Umeki, Nobuyuki Ito, Akira Yamada, Jun–Ichiro Miyagawa, Shigeki Higashiyama, Takao Iwawaki, Michiko Saito, Naoyuki Taniguchi, Yuji Matsuzawa, Kenji Kohno Gastroenterology Volume 124, Issue 3, Pages 701-707 (March 2003) DOI: 10.1053/gast.2003.50097 Copyright © 2003 American Gastroenterological Association Terms and Conditions

Fig. 1 Immunohistochemical detection of proHB-EGF in the liver of transgenic mice. (A) Sections of proHB-EGF transgenic mice were stained with an antibody (H1). (B) Nonimmunoreactivity can be seen in the control. For a negative control, the primary antiserum, antibody H1, was replaced by normal rabbit serum. Gastroenterology 2003 124, 701-707DOI: (10.1053/gast.2003.50097) Copyright © 2003 American Gastroenterological Association Terms and Conditions

Fig. 2 Light micrographs showing BrdU–labeled hepatocytes in the remnant livers of wild-type and transgenic mice at 48 hours after partial hepatectomy. (A) Low-power view, and (B) high-power view of the section of the HB-EGF transgenic mice liver at 48 hours after 70% partial hepatectomy. (C) Low-power view, and (D) high-power view of the section of the wild-type liver at 48 hours after 70% partial hepatectomy. Two hours before animals were killed, BrdU was administered intraperitoneally. DNA synthesis was detected by BrdU uptake and after immunochemical staining by using a monoclonal antibody to BrdU. Gastroenterology 2003 124, 701-707DOI: (10.1053/gast.2003.50097) Copyright © 2003 American Gastroenterological Association Terms and Conditions

Fig. 3 BrdU-labeling indexes of hepatocytes in wild-type and HB-EGF transgenic mice. Anti–BrdU-positive nuclei of hepatocytes as determined in 6000 hepatocytes for each mouse showing enhanced hepatocyte proliferation in the HB-EGF transgenic animals. Data represent means ± SE. ▩, wild type; ■, transgenic. Gastroenterology 2003 124, 701-707DOI: (10.1053/gast.2003.50097) Copyright © 2003 American Gastroenterological Association Terms and Conditions

Fig. 4 (A) Activities of MAP kinase after partial hepatectomy in the liver of HB-EGF transgenic (Tg) mice and wild-type (WT) mice. Remnant liver was removed at the indicated time after partial hepatectomy. Thirty-microgram liver lysates were analyzed by immunoblotting with anti-phospho pp42/44 MAP kinase and anti ERK2 as described in the Materials and Methods section. (B) Immunoblot analysis of mature HB-EGF. Remnant liver was removed at the indicated time after partial hepatectomy. Thirty-microgram liver lysates and 1 μg of recombinant mature HB-EGF (left lane) were fractionated by SDS–polyacrylamide gel electrophoresis and immunoblotted using the polyclonal HB-EGF antibody as described in the Materials and Methods section. Gastroenterology 2003 124, 701-707DOI: (10.1053/gast.2003.50097) Copyright © 2003 American Gastroenterological Association Terms and Conditions