Characterization of the first report of Mycobacterium timonense infecting an HIV patient in an Ecuadorian hospital J. Zurita, D. Ortega-Paredes, M. Mora,

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Characterization of the first report of Mycobacterium timonense infecting an HIV patient in an Ecuadorian hospital J. Zurita, D. Ortega-Paredes, M. Mora, N. Espinel, H. Parra, L. Febres, C. Zurita-Salinas Clinical Microbiology and Infection Volume 20, Issue 12, Pages O1113-O1116 (December 2014) DOI: 10.1111/1469-0691.12675 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Results of different identification methods used in strain NTMZ&Z1. (a) Thin layer growth pattern. (b) Matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) spectra. (c) PCR-RFLP analysis (PRA) pattern; L1, 25 bp ladder; L2, 1 kb ladder; BstEII digestion profile: 1, 231 bp; 2, 116 bp and 3, 94 bp fragments; HaeIII digestion profile: 4, 145 bp; 5, 127 bp; 6, 59 bp; 7, 40 bp, 36 bp and 34pb fragments. These fragments are the result of digestion of two separate aliquots of the 441pb hsp65 PCR fragment. (d) 2542pb concatenated sequences; rrs (1420pb), rpoB (707pb) and hsp65 (407pb) unweighted pair group method with arithmetic mean (UPGMA) tree. Clinical Microbiology and Infection 2014 20, O1113-O1116DOI: (10.1111/1469-0691.12675) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions