MicroRNA expression profiles in human testicular tissues of infertile men with different histopathologic patterns Masood Abu-Halima, M.Sc., Christina.

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MicroRNA expression profiles in human testicular tissues of infertile men with different histopathologic patterns Masood Abu-Halima, M.Sc., Christina Backes, Ph.D., Petra Leidinger, Ph.D., Andreas Keller, Prof.Dr., Abdel Monem Lubbad, M.D., Mohamad Hammadeh, Prof.Dr., Eckart Meese, Prof.Dr. Fertility and Sterility Volume 101, Issue 1, Pages 78-86.e2 (January 2014) DOI: 10.1016/j.fertnstert.2013.09.009 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Verification of miRNA level by quantitative real-time qRT-PCR of 5 differentially expressed microRNAs. (A) Sertoli cell only syndrome (SCO; n = 12); (B) mixed atrophy (MA; n = 12), (C) germ cell arrest (GA; n = 16); validated microRNAs. MicroRNAs are listed on the x-axis, and the y-axis refers to the relative expression levels ≥2.0-fold difference and 5% false-discovery rate. Fertility and Sterility 2014 101, 78-86.e2DOI: (10.1016/j.fertnstert.2013.09.009) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Venn diagram showing the overlap of miRNAs that were found to be down-regulated and up-regulated in Sertoli cell only syndrome (SCOS), mixed atrophy (MA), and germ cell arrest (GA) groups compared with that from normal control group (N). Diagram created with the use of Venny (http://bioinfogp.cnb.csic.es/tools/venny/index.html). Fertility and Sterility 2014 101, 78-86.e2DOI: (10.1016/j.fertnstert.2013.09.009) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Visualization of the microRNA microarray data of differentially expressed microRNAs between the samples. (A) Heat map of microRNAs correlating with mixed atrophic cell content (MA) vs. normal. (B) Heat map of microRNAs correlating with Sertoli cell only cell content (SCO) vs. normal. (C) Heat map of microRNAs correlating with germ cell arrest cell content (GA) vs. normal. (D) Clustering of 50 microRNAs with the highest variances; SCOS, MA, GA, and normal samples. Complete linkage hierarchic clustering was performed with the euclidian distance measure. The colors in the heatmap represent the normalized values, with lower values being colored in shades of green and higher values in shades of red. Fertility and Sterility 2014 101, 78-86.e2DOI: (10.1016/j.fertnstert.2013.09.009) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions