International Journal of Cardiology

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DOI: /j.atherosclerosis

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Respiration 2012;83:74–80 - DOI: /

Increased amount of bone marrow-derived smooth muscle-like cells and accelerated atherosclerosis in diabetic apoE-deficient mice J.O. Fledderus, O. van.

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The bone marrow-derived endothelial progenitor cell response is impaired in delayed wound healing from ischemia Stephen M. Bauer, MD, Lee J. Goldstein,

International Journal of Cardiology

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International Journal of Cardiology

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Endothelial nitric oxide synthase affects both early and late collateral arterial adaptation and blood flow recovery after induction of hind limb ischemia.

Differential expression of functional chemokine receptors on human blood and lung group 2 innate lymphoid cells Cathryn A. Weston, PhD, Batika M.J. Rana,

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E3 Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1α to Maintain Regulatory T Cell Stability and Suppressive Capacity Jee H. Lee, Chris Elly,

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International Journal of Cardiology The role of CD27-CD70-mediated T cell co-stimulation in vasculogenesis, arteriogenesis and angiogenesis K.H. Simons, Z. Aref, H.A.B. Peters, S.P. Welten, A.Y. Nossent, J.W. Jukema, J.F. Hamming, R. Arens, M.R. de Vries, P.H.A. Quax International Journal of Cardiology Volume 260, Pages 184-190 (June 2018) DOI: 10.1016/j.ijcard.2018.02.015 Copyright © 2018 The Authors Terms and Conditions

Fig. 1 Blood flow recovery after induction of hind limb ischemia. a. Paw perfusion was measured before and after surgery and 3, 7, 10, 13, 21 and 28 days after surgery. Control mice (n = 11, light blue), CD80/86−/− mice (n = 11, green), CD70−/− mice (n = 7, orange), CD70/80/86−/− mice (n = 8, red) and in CTLA-4+/− mice (n = 11, dark blue) were sacrificed after 28 days. b. Paw perfusion was measured before and after surgery and 3, 7 and 10 days after surgery. Control mice (n = 9), CD80/86−/− mice (n = 10), CD70−/− mice (n = 11), CD70/80/86−/− mice (n = 10) and in CTLA-4+/− mice (n = 6) were sacrificed after 10 days. Paw perfusion is expressed as a ratio of left (ischemic) to right (non-ischemic) paw perfusion. Data is presented as mean SEM; *p < 0.05; **p < 0.01. International Journal of Cardiology 2018 260, 184-190DOI: (10.1016/j.ijcard.2018.02.015) Copyright © 2018 The Authors Terms and Conditions

Fig. 2 Pre-existing collateral density in pial circulation. a. Representative image of pre-existing collaterals indicated by white stars (*) in pial circulation in control, CD80/86−/−, CD70/80/86−/− and CD70−/− mice. b. Total number of pre-existing collaterals per mm2 in pial circulation is shown in control mice (n = 5), CD80/86−/− mice (n = 7), CD70−/− mice (n = 7), CD70/80/86−/− mice (n = 5). Data are calculated as mean SEM; *p < 0.05. International Journal of Cardiology 2018 260, 184-190DOI: (10.1016/j.ijcard.2018.02.015) Copyright © 2018 The Authors Terms and Conditions

Fig. 3 Angiogenesis in soleus muscles. a. Representative image of CD31 (green) and CD3 (magenta) immunofluorescent double staining in soleus muscles is shown with DAPI (blue). b. representative image of CD31 staining in soleus muscle of a control mice is shown which was used for quantification (20× magnification) c. Quantification as L/R (left ischemic/right non-ischemic) ratio of the number of CD31 positive cells in soleus muscles is shown in control mice (n = 9), CD80/86−/− mice (n = 10), CD70−/− mice (n = 11), CD70/80/86−/− mice (n = 10) and CTLA-4+/− mice (n = 6) 10 days after surgery, d. and 28 days after surgery of control mice (n = 11), CD80/86−/− mice (n = 11), CD70−/− mice (n = 7), CD70/80/86−/− mice (n = 8) and CTLA-4+/− mice (n = 11) and in e. both 10 and 28 days after surgery. Data are calculated as the ratio of L/R and presented as mean SEM; *p < 0.05; **p < 0.01, ***p < 0.001. International Journal of Cardiology 2018 260, 184-190DOI: (10.1016/j.ijcard.2018.02.015) Copyright © 2018 The Authors Terms and Conditions

Fig. 4 Arteriogenesis in adductor muscles. a. Representative image of αSMActin staining in adductor muscle tissue (20× magnification). b. Number of collateral arterioles is shown in the left paw 10 days after surgery in control (n = 9), CD80/86−/− (n = 10), CD70−/− (n = 11), CD70/80/86−/− (n = 10) and in CTLA-4+/− mice (n = 6). c. Number of collateral arterioles is shown in the left paw 28 days after surgery in control (n = 11), CD80/86−/− (n = 11), CD70−/− (n = 7), CD70/80/86−/− (n = 8) and in CTLA-4+/− mice (n = 11). d. Diameter of αSMActin positive collateral arterioles, presented as L/R (left ischemic/right non-ischemic) ratio, is shown 10 days after surgery, e. 28 days after surgery and f. in both 10 and 28 days after surgery. Data are presented as mean SEM; *p < 0.05; **p < 0.01, ***p < 0.001. International Journal of Cardiology 2018 260, 184-190DOI: (10.1016/j.ijcard.2018.02.015) Copyright © 2018 The Authors Terms and Conditions

Fig. S1 Percentage of KLRG1+ CD62L−, CD4+ or CD8+ T cells. Blood, bone marrow, lymph node and spleen were analyzed with FACS of control CD80/86−/−, CD70−/−, CD70/80/86−/− and CTLA4+/− mice, sacrificed 28 days after surgery. a. CD4+ T cells b. CD8+ T cells. n = 3–6. Data is presented as mean SEM; *p < 0.05, **p < 0.01. International Journal of Cardiology 2018 260, 184-190DOI: (10.1016/j.ijcard.2018.02.015) Copyright © 2018 The Authors Terms and Conditions

Fig. S2 Percentage of KLRG1+ CD62L−, CD4+ or CD8+ T cells. Blood, bone marrow, lymph node and spleen were analyzed with FACS of control CD80/86−/−, CD70−/−, CD70/80/86−/− and CTLA4+/− mice, sacrificed 10 days after surgery. a. CD4+ T cells b. CD8+ T cells. n = 3–6. Data is presented as mean SEM; *p < 0.05, **p < 0.01. International Journal of Cardiology 2018 260, 184-190DOI: (10.1016/j.ijcard.2018.02.015) Copyright © 2018 The Authors Terms and Conditions

Fig. S3 T cell activation via VSMC measured in control, CD80/86−/−, CD70−/− and CD70/80/86−/− VSMCs. T cells of an OT-I mouse were added to the cultured VSMCs together with Ova and LPS (100 ng/ml). IFNy (in pg/ml) concentration was measured in supernatant of VSMCs after 24 h. CD80/86−/−, CD70−/− and CD70/80/86−/− n = 4, control n = 10. Data are calculated as mean SEM; **p < 0.01. International Journal of Cardiology 2018 260, 184-190DOI: (10.1016/j.ijcard.2018.02.015) Copyright © 2018 The Authors Terms and Conditions

Fig. S4 Diameter of αSMActin positive collateral arterioles divided in <20 μm2 and >20 μm2. Data presented as L/R (left ischemic/right non-ischemic) ratio. a. 10 days after surgery ischemic/non-ischemic ratio of number of collateral arterioles <20 μm2 and b. >20 μm2 was quantified. c. and also 28 days after surgery number of collateral arterioles <20 μm2 and d. >20 μm2 was quantified. International Journal of Cardiology 2018 260, 184-190DOI: (10.1016/j.ijcard.2018.02.015) Copyright © 2018 The Authors Terms and Conditions