Critical Evaluation of Real-Time Reverse Transcriptase-Polymerase Chain Reaction for the Quantitative Detection of Cytokeratin 20 mRNA in Colorectal Cancer.

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Presentation transcript:

Critical Evaluation of Real-Time Reverse Transcriptase-Polymerase Chain Reaction for the Quantitative Detection of Cytokeratin 20 mRNA in Colorectal Cancer Patients Nadia Dandachi, Marija Balic, Stefanie Stanzer, Michael Halm, Margit Resel, Thomas Anton Hinterleitner, Hellmut Samonigg, Thomas Bauernhofer The Journal of Molecular Diagnostics Volume 7, Issue 5, Pages 631-637 (November 2005) DOI: 10.1016/S1525-1578(10)60597-1 Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Box plots show that expression of PBGD was not significantly different among HVs, patients with CRC (Pat), and patients with CID (P = 0.100; Kruskal-Wallis test). The Journal of Molecular Diagnostics 2005 7, 631-637DOI: (10.1016/S1525-1578(10)60597-1) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 ROC curve for CK20 mRNA ratios to distinguish CRC patients (n = 82) from HVs (n = 37). ROC curves were constructed by plotting sensitivity and 100-specificity corresponding to each cutoff value for CK20 mRNA. The cutoff of 415 gave a sensitivity of 55% and a specificity of 78%. Area under the curve = 0.672; SE = 0.051; 95% confidence range, 0.580–0.756; P < 0.003. The Journal of Molecular Diagnostics 2005 7, 631-637DOI: (10.1016/S1525-1578(10)60597-1) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Comparison of CK20 mRNA ratio between patients with CRC, LCC, and MCC; HVs; and patients with CID. Black bars, median; Cutoff value 415 is indicated by dashed horizontal line. The Journal of Molecular Diagnostics 2005 7, 631-637DOI: (10.1016/S1525-1578(10)60597-1) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Representative results of a CK20 qRT-PCR using the LightCycler. Solid lines indicate amplification curves from the target gene (CK20) and dashed lines from the reference gene (PBGD). No product was amplified in the no-template sample or when reverse transcription was omitted (•). The Journal of Molecular Diagnostics 2005 7, 631-637DOI: (10.1016/S1525-1578(10)60597-1) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions