The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility.

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Presentation transcript:

The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility prognostic factor Marcos Meseguer, Ph.D., J.A. Martínez-Conejero, Ph.D., J. Enrique O'Connor, Ph.D., Antonio Pellicer, M.D., José Remohí, M.D., Nicolás Garrido, Ph.D. Fertility and Sterility Volume 89, Issue 5, Pages 1191-1199 (May 2008) DOI: 10.1016/j.fertnstert.2007.05.005 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Graphic representation of the relationship between fresh and capacitated percentage of sperm with DNA oxidation and zygote patterns. A representative figure of each type of classified embryo has been included in the graphic. ∗Significant increase of the percentage of oxidized DNA sperm cells on type IV pattern compared with other three patterns (P<.05). Fertility and Sterility 2008 89, 1191-1199DOI: (10.1016/j.fertnstert.2007.05.005) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Percentage of cells with oxidized DNA with respect to classification of embryos on day 3 depending on blastomeres symmetry. We defined three types of embryos: type I: embryo cell volume with equal distribution, this means similar size in even blastomere–number embryos, i.e., four, six, or eight cells, or one cell with bigger diameter in odd blastomere–number embryos; type III: embryos with one big cell considered to be dominant and which represents at least one-third of the embryo volume; and type II: embryos with odd blastomere number of equal sizes and embryos with even or odd numbers but different blastomere sizes. A representative picture of each type of classified embryo has been included in the graphic. ∗Significant difference of the percentage of oxidized DNA sperm cells between the types considered (P<.05). Fertility and Sterility 2008 89, 1191-1199DOI: (10.1016/j.fertnstert.2007.05.005) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Classifications of blastocyst as those which developed blastocoel cavity on day 6 (144 hours after fertilization) compared with the rest of the embryos that did not reach the blastocyst stage, which were considered to be arrested. A representative picture of both types of classified embryos has been included in the graphic. A comparison of the percentage of sperm cells with oxidized DNA and the blastocysts and arrested embryos was performed. ∗Significant difference of the percentage of oxidized DNA fresh sperm cells between both types considered (P<.05). Fertility and Sterility 2008 89, 1191-1199DOI: (10.1016/j.fertnstert.2007.05.005) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Classification of the embryos into three categories depending on the final destination: nonviable, frozen, and transfered embryos. Results from the compilation of these categories with OxiDNA shown that increased DNA oxidation is associated with nonviable (bad-quality) embryos. ∗Significant increase in the percentage of oxidized DNA sperm cells in those nonviable embryos produced compared with frozen and transfered (good-quality) embryos (P<.05). Fertility and Sterility 2008 89, 1191-1199DOI: (10.1016/j.fertnstert.2007.05.005) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions