OmpA C-Terminus Library

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OmpA C-Terminus Library 5uL of ligation reaction (4500 !! ng DNA) were transformed into chemically competent BL29 Gold DE3 Cells. 5uL 50uL 200uL Vector 2 33 lots 10mer 14 149 15mer 6 275 uL transformant vs. colony count

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Biobricking the Fec System Mutagenesis of Fec promoter to weaken gene expression, providing a range of sensitivity. Mutagenesis of the Fec promoter to remove FUR repressor binding site, allowing easier assays. Studies detailing the response of reporter expression to mutations at each site of the promoter have been done. (Enz, Pressler, Braun papers)