Dissemination of multidrug-resistant, class 1 integron-carrying Acinetobacter baumannii isolates in Taiwan  L.-Y. Huang, T.-L. Chen, P.-L. Lu, C.-A. Tsai,

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Dissemination of multidrug-resistant, class 1 integron-carrying Acinetobacter baumannii isolates in Taiwan  L.-Y. Huang, T.-L. Chen, P.-L. Lu, C.-A. Tsai, W.-L. Cho, F.-Y. Chang, C.-P. Fung, L.K. Siu  Clinical Microbiology and Infection  Volume 14, Issue 11, Pages 1010-1019 (November 2008) DOI: 10.1111/j.1469-0691.2008.02077.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Schematic representation of the variable regions identified in multidrug-resistant Acinetobacter baumannii (MDR-AB) isolates. Cassette types (1–7) are arranged as identified in Table 2. Gene cassettes are shown as boxes, with arrows indicating the orientation of transcription and black circles indicating the 59-base elements. Hatched rectangles indicate 5′- and 3′-conserved segments (CSs). The 5′-CS and 3′-CS amplification primers are indicated by arrowheads. Clinical Microbiology and Infection 2008 14, 1010-1019DOI: (10.1111/j.1469-0691.2008.02077.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 (a) Pulsed-field gel electrophoresis (PFGE) analysis by ApaI restriction enzyme digestion of 43 Acinetobacter baumannii isolates with ribotype (R)5 (left panel) and R6 (right panel). Isolates from different geographical areas are shown as follows: lane 1, R25 control endemic clone from another hospital in Taiwan [11]; lanes 2–15, northern Taiwan R5 isolates; lanes 16–23, central Taiwan R5 isolates; lanes 24–28, southern Taiwan R5 isolates; lanes 29–39, northern Taiwan R6 isolates; lane 40, central Taiwan R6 isolate; lanes 41–43, southern Taiwan R6 isolates; lane M, molecular weight marker, (b) Dendrogram generated by UPGMA clustering. Isolates with similarity of >90% were considered to be identical; isolates with <70% similarity (>3 band difference) were considered to be non-clonally related. Tol, tolerance; OPT, optimum; Min, minimum; UPGMA, unweighted pair group method using arithmetic averages. Clinical Microbiology and Infection 2008 14, 1010-1019DOI: (10.1111/j.1469-0691.2008.02077.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions