Reduction in Induced Pain by Ultrasound May Be Caused by Altered Expression of Spinal Neuronal Nitric Oxide Synthase-Producing Neurons Yueh-Ling Hsieh,

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Reduction in Induced Pain by Ultrasound May Be Caused by Altered Expression of Spinal Neuronal Nitric Oxide Synthase-Producing Neurons Yueh-Ling Hsieh, PT, PhD Archives of Physical Medicine and Rehabilitation Volume 86, Issue 7, Pages 1311-1317 (July 2005) DOI: 10.1016/j.apmr.2004.12.035 Copyright © 2005 American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation Terms and Conditions

Fig 1 Serial changes of locomotor activity in (A) HA and (B) VA monitored at 1 day before CFA injection (Before), 4 hours after CFA injection (CFA), and immediately after sham-operated and US treatments (Treatment). Archives of Physical Medicine and Rehabilitation 2005 86, 1311-1317DOI: (10.1016/j.apmr.2004.12.035) Copyright © 2005 American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation Terms and Conditions

Fig 2 Photomicrographs showing nNOS-LI neurons in the L2 segment ipsilateral to the CFA-injected leg after (A) sham-operated and (B) US treatments. (C) The leg processed with thionin Nissl stain for identifying the location of nNOS-LI neurons. NOTE. The length of each scale bar is 0.1mm. Archives of Physical Medicine and Rehabilitation 2005 86, 1311-1317DOI: (10.1016/j.apmr.2004.12.035) Copyright © 2005 American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation Terms and Conditions

Fig 3 The rostrocaudal extent of nNOS-LI in the L2 segment of 2 CFA-induced arthritic rats treated with sham or US. The arrows indicate the ipsilateral to the injected leg. Archives of Physical Medicine and Rehabilitation 2005 86, 1311-1317DOI: (10.1016/j.apmr.2004.12.035) Copyright © 2005 American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation Terms and Conditions

Fig 4 Histograms of the effect of sham-operated and US treatment in animals with CFA-induced arthritis on numbers of nNOS-LI neurons of the L1-2 segments of rat spinal cord ipsilateral (IPSI) and contralateral (CONTRA) to the CFA-injected leg. Each value represents the average number of labeled neurons ± SEM in each group for each laminar distribution. *P<.05; **P<.01; ***P<.001. Archives of Physical Medicine and Rehabilitation 2005 86, 1311-1317DOI: (10.1016/j.apmr.2004.12.035) Copyright © 2005 American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation Terms and Conditions

Fig 5 The spatial distribution proportion (in percent) of nNOS-LI neurons in laminae I–II, III–IV, V–VI, and VII–X in CFA-induced arthritis animals sham-treated or treated with US ipsilateral and contralateral to the injected legs. Significant differences are shown between sham-treated rats and those treated with US in ipsilateral laminae III–IV and laminae V–VI. **P<.01; ***P<.001. Archives of Physical Medicine and Rehabilitation 2005 86, 1311-1317DOI: (10.1016/j.apmr.2004.12.035) Copyright © 2005 American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation Terms and Conditions