Redesign of a Dioxygenase in Morphine Biosynthesis

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Redesign of a Dioxygenase in Morphine Biosynthesis Weerawat Runguphan, Weslee S. Glenn, Sarah E. O'Connor Chemistry & Biology Volume 19, Issue 6, Pages 674-678 (June 2012) DOI: 10.1016/j.chembiol.2012.04.017 Copyright © 2012 Elsevier Ltd Terms and Conditions

Chemistry & Biology 2012 19, 674-678DOI: (10. 1016/j. chembiol. 2012 Copyright © 2012 Elsevier Ltd Terms and Conditions

Figure 1 Biosynthesis of Benzylisoquinoline Alkaloids in P. somniferum The biosynthetic pathways leading to morphine 11 via morphinone 9 and codeine 10. Chemistry & Biology 2012 19, 674-678DOI: (10.1016/j.chembiol.2012.04.017) Copyright © 2012 Elsevier Ltd Terms and Conditions

Figure 2 Homology Models of PsT6ODM and PsCODM Based on the Crystal Structure of AtANS PsT6ODM is indicated in green, and PsCODM is indicated in pink. The models were created using SWISS-MODEL. Regions A1 and A2 (residues 145–152) are shown in red, B (residues 334–336) is in yellow, and C1 and C2 (residues 338–346) are in blue. See also Figures S1, S2, S3, and S4 and Tables S1 and S2. Chemistry & Biology 2012 19, 674-678DOI: (10.1016/j.chembiol.2012.04.017) Copyright © 2012 Elsevier Ltd Terms and Conditions

Figure 3 Biochemical Activity of PsCODM Enzymes LC-MS traces of an in vitro enzymatic assay of wild-type and selected mutant PsCODM enzymes (1 μM final enzyme concentration) with 0.25 mM thebaine 5 and 0.25 mM codeine 10 in Tris buffer (100 mM Tris-HCl, 10% [v/v] glycerol, 14 mM 2-mercaptoethanol, pH 7.4) containing 10 mM 2-oxoglutarate, 10 mM sodium ascorbate and 0.5 mM iron (II) sulfate. Reactions were performed at 30°C for 4 hr. Mass-to-charge ratio (m/z) 286 corresponds to morphine 11; m/z 298 corresponds to oripavine 6. LC-MS spectra are normalized to the same y axis scale. See also Figures S1, S2, S3, and S4 and Tables S1 and S2. Chemistry & Biology 2012 19, 674-678DOI: (10.1016/j.chembiol.2012.04.017) Copyright © 2012 Elsevier Ltd Terms and Conditions