Mark Gorelik, MD, Satya D. Narisety, MD, Anthony L

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Suppression of the immunologic response to peanut during immunotherapy is often transient Mark Gorelik, MD, Satya D. Narisety, MD, Anthony L. Guerrerio, MD, PhD, Kristin L. Chichester, MS, Corinne A. Keet, MD, MD, Anja P. Bieneman, BS, Robert G. Hamilton, PhD, Robert A. Wood, MD, John T. Schroeder, PhD, Pamela A. Frischmeyer-Guerrerio, MD, PhD Journal of Allergy and Clinical Immunology Volume 135, Issue 5, Pages 1283-1292 (May 2015) DOI: 10.1016/j.jaci.2014.11.010 Copyright © 2015 Terms and Conditions

Fig 1 SHR and constitutive expression of basophil activation markers during immunotherapy. SHR (A), constitutive CD63 expression (B), and CD203c mean fluorescence intensity (MFI; C) were measured in basophil-enriched suspensions from subjects undergoing OIT (left panels) and SLIT (right panels) for peanut. T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig 2 Effect of OIT and SLIT on basophil CD63 expression and HR in response to peanut. Basophil-enriched suspensions were stimulated with crude peanut extract at 0.1 ng/mL (A), 1 ng/mL (B), and 10 ng/mL (C). SHR and CD63 expression in medium alone were subtracted to obtain stimulated values. T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig 3 Changes in basophil IL-4 expression during peanut OIT and SLIT. Basophil expression of IL-4 was measured by using intracellular flow cytometry after incubation of whole-blood samples with peanut (A), anti-IgE (B), dust mite at 1 AU/mL (C) or 10 AU/mL (D), and ionomycin (E). T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig 4 Effect of peanut OIT and SLIT on TH2 cytokine responses in DC–T-cell cocultures. IL-5 and IL-13 were measured in supernatants from cocultures of pDCs and mDCs with autologous CD4+ T cells stimulated with 50 μg/mL crude peanut extract (A) or 100 AU/mL dust mite (B). Spontaneous cytokine secretion, as measured in medium alone, was subtracted to obtain allergen-induced values. T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig 5 Effect of peanut OIT and SLIT on DC expression of costimulatory molecules and HLA-DR. Mean fluorescence intensity (MFI) of CD40, CD86, and HLA-DR on pDCs (left 2 columns) or mDCs (right 2 columns) was measured after coculture with CD4+ T cells stimulated with 50 μg/mL peanut (A) or 100 AU/mL dust mite (B). T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig 6 Effect of peanut OIT and SLIT on expression of CD80 on mDCs. CD80 mean fluorescence intensity (MFI) was measured on mDCs after coculture with CD4+ T cells stimulated with media alone (A), 50 μg/mL peanut (B), or 100 AU/mL dust mite (C). T1 to T7 correspond to time points at which blood was collected. OIT: n = 7 at T1 to T5, n = 5 at T6, and n = 4 at T7. SLIT: n = 8 at T1 to T4, n = 7 at T5, n = 8 at T6, and n = 4 at T7. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig E1 Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

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Fig E3 Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig E4 Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

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Fig E8 Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig E9 Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions

Fig E10 Journal of Allergy and Clinical Immunology 2015 135, 1283-1292DOI: (10.1016/j.jaci.2014.11.010) Copyright © 2015 Terms and Conditions