Volume 80, Issue 5, Pages (May 2001)

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Volume 80, Issue 5, Pages 2262-2272 (May 2001) Effects of Lung Surfactant Proteins, SP-B and SP-C, and Palmitic Acid on Monolayer Stability  Junqi Ding, Dawn Y. Takamoto, Anja von Nahmen, Michael M. Lipp, Ka Yee C. Lee, Alan J. Waring, Joseph A. Zasadzinski  Biophysical Journal  Volume 80, Issue 5, Pages 2262-2272 (May 2001) DOI: 10.1016/S0006-3495(01)76198-X Copyright © 2001 The Biophysical Society Terms and Conditions

Figure 1 (A) Quasi-static cycling isotherms (compression–expansion–compression) for a DPPC/POPG/PA, 68:22:8 (wt/wt/wt) lipid mixture on a buffered saline subphase (150mM NaCl, 5mM CaCl, 0.2mM NaHCO3, pH=6.9) at 30°C. There were no significant variations in any of the isotherms over the temperature range of 30–37°C. Without protein, the second compression shows a significant offset in area per molecule likely due to loss of the unsaturated POPG to the subphase. Subsequent compressions show that the POPG is irreversibly lost and the maximum surface pressure steadily decreases. Surface pressure (reduction in surface tension from the clean interface) is plotted versus the area per lipid molecule in A–C. (B) Cycling isotherms of the same lipid mixture and subphase as (A), but with 2wt % SP-Cff added at 30°C. The plateau in the isotherm at a surface pressure of ∼50mN/m is obviouson the first compression. The second and subsequent compressions show a small break in the isotherm at the same surface pressure as the original plateau and the collapse pressure increases to >70mN/m. The offset in area per molecule is diminished, suggesting that less POPG is lost to the subphase. (C) Cycling isotherms of 3:1 DPPC/POPC with 2wt % SP-Cff at 30°C. Instead of the small break in the isotherm at ∼50mN/m (B), there is a distinct plateau at a pressure of 40mN/m, and another break in the isotherm at ∼55mN/m on the second and subsequent compressions. Without PA, the surface pressure for a given compression is less than with PA (B). There is even less offset in area per lipid molecule between the first and second cycles in the presence of PA (B). (D) Cycling isotherms of Survanta on the same subphase as (A, B) at 30°C. The first compression shows a very similar break in the isotherm as in (B), which is retained on subsequent compressions. There appears to be almost no offset in area per molecule after the first compression. (E) Cycling isotherms of Survanta with 5wt % added SP-B1–25 peptide at collapse at 37°C. The isotherm is quite similar to that in D. The peptide partitions mainly into the fluid phase domains and does not affect the solid phase domains to any great extent. Biophysical Journal 2001 80, 2262-2272DOI: (10.1016/S0006-3495(01)76198-X) Copyright © 2001 The Biophysical Society Terms and Conditions

Figure 2 (A) Contact mode AFM image of model lung surfactant monolayer (DPPC/POPG/PA/SP-Cff in weight ratio of 68:22:8:2) transferred from the air-water interface at a surface pressure just below the break in the isotherm (45mN/m, see Fig. 1 B) onto a mica substrate (Lee et al., 1998) at 30°C. The height trace (white line in image) shows the solid phase domains (light gray) are 1–2nm thicker than the fluid phase domains (dark gray). The solid phase domains consist of all trans, extended chains (Lee et al., submitted for publication) and are expected to be thicker and less compressible than the fluid phase domains, which consist of disordered chains (Marsh, 1990). The irregular shape and 5–10 micron size of the solid phase domains are the same as observed at the air-water interface with fluorescence microscopy. (B) Contact mode AFM image of the same film (DPPC/POPG/PA/SP-Cff in weight ratio of 68:22:8:2) transferred at a surface pressure of 55mN/m at 30°C, which is above the break in the isotherm. Now, the fluid phase domains are light gray, indicating that the fluid phase is thicker than the solid phase (dark gray). The height trace (white line in image) shows that the fluid phase is ∼4nm higher than the solid phase. This shows that the fluid phase has increased in thickness by 5–6nm in comparison to Fig. 2 A. (C) AFM image of 3:1 DPPC/POPC, with 2wt % SP-Cff transferred at a surface pressure above the first plateau in the isotherm at 50mN/m and 30°C. When the solid phase consists of only DPPC, the fluid phase forms multilayer steps rather than a uniformly thickened fluid phase (B). This stepped morphology is similar to that observed in DPPC/DPPG films with palmitoylated human recombinant SP-C (von Nahmen et al., 1997). (D) AFM image of Survanta monolayer transferred at a surface pressure of 45mN/m at 30°C, just below the plateau in the isotherm. The fluid phase (dark gray) is 1–2nm lower than the solid phase domainsas shown on the height trace. The bright white specks across the image are small aggregates of Survanta dispersed in the aqueous phase that were trapped during transfer. (E) AFM image of Survanta monolayer transferred at a surface pressure of 55mN/m and 30°C, just above the plateau in the isotherm. The solid phase is now dark gray and the fluid phase is lighter gray. As in B, the fluid phase domains have thickened, but here they are only 1–2nm higher than the solid phase domains. As the amount of thickening of the fluid domains scales with the amount of SP-C present, this may be consistent with a smaller fraction of SP-C in Survanta than in the model films. Overall, the general features of Survanta films appear very similar to the DPPC/POPG/PA/SP-Cff films shown in B. Biophysical Journal 2001 80, 2262-2272DOI: (10.1016/S0006-3495(01)76198-X) Copyright © 2001 The Biophysical Society Terms and Conditions

Figure 3 The speed of a magnetic needle floating on a surfactant-covered interface at a given magnetic force was measured as a function of surface pressure for DPPC/POPG (77:23, wt/wt) monolayers with differing PA content on a pure water subphase at 25°C. The speed is normalized to the needle velocity on a clean, surfactant-free interface. A high needle velocity corresponds to a low monolayer viscosity, although it is difficult to assign a single value to the viscosity of a non-Newtonian monolayer. With no PA in the monolayer, the needle velocity is nearly independent of surface pressure and is not significantly different from a clean interface (normalized speed of 1). For a PA fraction of 10%, there is a transition between 30 and 40mN/m from high needle velocity to low needle velocity. This change in the monolayer mechanical properties correlates with a change in the morphological transitions in Fig. 2 and with the collapse structures seen in Fig. 4. For 20wt % PA, the monolayer is much stiffer, and by 20mN/m, we cannot drive the needle across the interface with the maximum force we can apply. For surface pressures >50mN/m, the needle often sinks and we cannot measure surface viscosity. Biophysical Journal 2001 80, 2262-2272DOI: (10.1016/S0006-3495(01)76198-X) Copyright © 2001 The Biophysical Society Terms and Conditions

Figure 4 (A) Fluorescence optical micrograph of coexisting monolayer and folded region in a film of DPPC/POPG/PA/SP-B1–78, in weight ratio 68:22:8:3 at the collapse pressure of 65mN/m at 37°C. The bright fluid phase network (likely containing POPG, the protein and the fluorescent lipid, which separates into disordered phases) separates the darker solid phase domains (probably containing the DPPC and PA) both in the monolayer and in the folded region (Lipp et al., 1998). The organization and distribution of the domains remains the same within the folds, implying that the composition also remains the same. The folds reincorporate into the monolayer on expansion over a range in surface pressure from the collapse pressure to ∼15mN/m. The folds always protrude into the subphase. Without SP-B the films do not fold, but rather fracture, as in Fig. 4B. (B) Fluorescence image of Survanta at 37°C at collapse showing a similar solid-fluid phase coexistence (see Fig. 4A), although with significantly smaller domains. At collapse, Survanta fractures to form bright cracks at the interface. The materials in and around the cracks are slow to respread on expansion of the monolayer. (C) Fluorescence image of Survanta with 5wt % added SP-B1–25 peptide at collapse at 37°C. As in Fig. 4B, there is solid-fluid phase coexistence with a small domain size, but theadded SP-B causes the monolayer to undergo a reversible folding as in the model mixture (Fig. 4A). The folds are somewhat smaller than in the model mixture and appear to scale with the domain size. The folded regions appear to have the same overall composition as the rest of the monolayer, and reincorporate readily into the monolayer on expansion. Biophysical Journal 2001 80, 2262-2272DOI: (10.1016/S0006-3495(01)76198-X) Copyright © 2001 The Biophysical Society Terms and Conditions