Quantitative assessment of articular cartilage and subchondral bone histology in the meniscectomized guinea pig model of osteoarthritis P Pastoureau,

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Presentation transcript:

Quantitative assessment of articular cartilage and subchondral bone histology in the meniscectomized guinea pig model of osteoarthritis P Pastoureau, Ph.D., S Leduc, M.Sc., A Chomel, B.Sc., F De Ceuninck, Ph.D. Osteoarthritis and Cartilage Volume 11, Issue 6, Pages 412-423 (June 2003) DOI: 10.1016/S1063-4584(03)00050-5

Fig. 1 Left operated proximal tibia of a meniscectomized guinea pig. Site of measurement of histomorphometric parameters: medial tibial plateau (arrow). Safranin O staining. OP, osteophyte; L, ligament; C, cyst; GP, growth plate (magnification: 10×). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 2 (A) Left operated tibial plateau of a meniscectomized guinea pig. Area of measurement of the CT (in μm): automatically generated between the two manually indicated points (1 and 2) at the margin of cartilage (C) and bone (B). Safranin O staining. OP, osteophyte (magnification: 20×). (B) Results of CT (mean ± s.e.m.) in SH and MNX animals, 1 and 3 months post-operation. Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 3 (A) Left operated tibial plateau of a SH and MNX guinea pig. Measurement of the FI; automatically calculated as follows: FI=(length of superficial margin of cartilage (l)/width of area of measurement (w))×100. Safranin O staining (magnification: 80×). (B) Results of FI (mean ± s.e.m.) in SH and MNX animals, 1 and 3 months post-operation (significant differences between SH and MNX: ∗∗P<0.01; ∗∗∗P<0.001; significant difference in SH between 1 and 3 months: # P<0.05). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 4 (A) Left operated tibial plateau of a SH and MNX guinea pig. Automatic measurement of the SOI in the deep (D) and superficial (S) zones of the articular cartilage after manual delimitation of the D from the S zones. Proteoglycan content ratio of the cartilage is expressed as SOI in S/SOI in D. Safranin O staining (magnification: 80×). (B) Results of proteoglycan content ratio (mean ± s.e.m.) in SH and MNX animals, 1 and 3 months post-operation (significant difference between SH and MNX: ∗∗∗P<0.001). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 5 (A) Left operated tibial plateau of a SH and MNX guinea pig. Measurement of the CD calculated as follows: number of cells (manual counting) divided by the automatically measurement of the central area of the medial tibial plateau (nb cells/mm2). Safranin O staining (magnification: 200×). (B) Results of CD (mean ± s.e.m.) in SH and MNX animals, 1 and 3 months post-operation (significant difference between SH and MNX: ∗∗∗P<0.001). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 6 (A) Left medial tibial plateau of a guinea pig. Bone volume (BV in %) and subchondral bone plate thickness (SBPT in μm) were automatically measured after segmentation of bone in the half superior part of the epiphysis (dark area). Goldner trichrome staining. L, ligament; GP, growth-plate; BE, bone epiphysis (magnification: 12.5×). (B) Results of BV and SBPT (mean ± s.e.m.) in SH and MNX animals, 1 and 3 months post-operation (significant differences between SH and MNX: ∗P<0.005; significant differences in SH between 1 and 3 months: #P<0.05). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 7 Pearson's (r) and Spearman's (r′) correlations between the CT and the subchondral bone plate thickness of the medial tibial plateau calculated at the two grouped time points for all the animals (∗∗P<0.01). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 8 Pearson's (r) and Spearman's (r′) correlations between the proteoglycan content ratio and the FI of the medial tibial plateau calculated at the two grouped time points for all the animals (∗∗P<0.01; ∗∗∗P<0.001). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 9 Pearson's (r) and Spearman's (r′) correlations between the CD and the FI of the medial tibial plateau calculated at the two grouped time points for all the animals (∗P<0.05; ∗∗∗P<0.001). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 10 Pearson's (r) and Spearman's (r′) correlations between the CD and the proteoglycan content ratio of the medial tibial plateau calculated at the two grouped time points for all the animals(∗∗P<0.01). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)

Fig. 11 Pearson's (r) and Spearman's (r′) correlations between the subchondral bone plate thickness and the bone volume of the medial tibial plateau calculated at the two grouped time points for all the animals (∗∗∗P<0.001; ∗P<0.05). Osteoarthritis and Cartilage 2003 11, 412-423DOI: (10.1016/S1063-4584(03)00050-5)