Volume 44, Issue 6, Pages 1175-1185 (June 2006) The diagnostic value of biomarkers (AshTest) for the prediction of alcoholic steato- hepatitis in patients with chronic alcoholic liver disease  Dominique Thabut, Sylvie Naveau, Frederic Charlotte, Julien Massard, Vlad Ratziu, Françoise Imbert-Bismut, Dominique Cazals-Hatem, Annie Abella, Djamila Messous, Fabienne Beuzen, Mona Munteanu, Julien Taieb, Richard Moreau, Didier Lebrec, Thierry Poynard  Journal of Hepatology  Volume 44, Issue 6, Pages 1175-1185 (June 2006) DOI: 10.1016/j.jhep.2006.02.010 Copyright © 2006 European Association for the Study of the Liver Terms and Conditions

Fig. 1 Flow sheet of patients analyzed and included in the training and validation groups. Journal of Hepatology 2006 44, 1175-1185DOI: (10.1016/j.jhep.2006.02.010) Copyright © 2006 European Association for the Study of the Liver Terms and Conditions

Fig. 2 Diagnostic values of AshTest, Maddrey and AST/ALT ratio for the diagnosis of alcoholic hepatitis. Panel A: Box plots of AshTest, Maddrey and AST/ALT ratio according to severity of alcoholic hepatitis (n=225). A four grades scoring system was used to assess alcoholic hepatitis: 0 none, 1–2 mild, 3–4 moderate, 5–8 severe. This score combines elementary features (necrosis, polymorphonuclear infiltrate, Mallory bodies and ballooning). Notched box plots show the relationship between the biomarkers and grade of alcoholic hepatitis in the integrated database. The horizontal line inside each box represents the median and the width of each box the median±1.57 interquartile range/n to assess the 95% level of significance between group medians. Failure of the shaded boxes to overlap signifies statistical significance (P<0.05). The horizontal lines above and below each box encompass the interquartile range (from 25th to 75th percentile), and the vertical lines from the ends of the box encompass the adjacent values (upper: 75th percentile plus 1.5 times interquartile range, lower 25th percentile minus 1.5 times interquartile range). Panel B: ROC curves of AshTest (solid black line), Maddrey (grey line) and AST/ALT ratio (dotted line) for the diagnosis of alcoholic hepatitis (n=225). Journal of Hepatology 2006 44, 1175-1185DOI: (10.1016/j.jhep.2006.02.010) Copyright © 2006 European Association for the Study of the Liver Terms and Conditions

Fig. 3 Relationship between AshTest, Maddrey discriminant function and AST/ALT ratio according to each elementary features of alcoholic hepatitis. Polymorphonuclear infiltrate: 0, none; 1, moderate; 2, marked; necrosis: 0, none; 1, presence; Mallory bodies: 0, none; 1, moderate; 2, marked; ballooning: 0, none; 1, moderate; 2, marked. Journal of Hepatology 2006 44, 1175-1185DOI: (10.1016/j.jhep.2006.02.010) Copyright © 2006 European Association for the Study of the Liver Terms and Conditions