Angiotensin type 2 receptors in the intermediolateral cell column of the spinal cord: Negative regulation of sympathetic nerve activity and blood pressure 

Slides:



Advertisements
Similar presentations
Date of download: 5/28/2016 Copyright © The American College of Cardiology. All rights reserved. From: Contractile Function During Angiotensin-II Activation:
Advertisements

Fig. 2. The expression of pKr-2 in the substantia nigra (SN) of patients with Parkinson's disease (PD). (A, B) Western blot analysis for pKr-1-2 and pKr-2,
Activation of Src Family Kinases in Spinal Microglia Contributes to Formalin-Induced Persistent Pain State Through p38 Pathway  Yong-Hui Tan, Kai Li,
The chemokine CCL2 activates p38 mitogen-activated protein kinase pathway in cultured rat hippocampal cells  Jungsook Cho, Donna L. Gruol  Journal of.
Volume 93, Pages 1-11 (December 2016)
Volume 15, Issue 5, Pages (May 2014)
Seizure - European Journal of Epilepsy
Dietary apigenin regulates high glucose and hypoxic reoxygenation-induced reductions in apelin expression in human endothelial cells  Kazuo Yamagata,
Mallahalli S. Manu, Kuruvanthe S. Rachana, Gopal M. Advirao
Nogo-p4 Suppresses TrkA Signaling Induced by Low Concentrations of Nerve Growth Factor Through NgR1 in Differentiated PC12 Cells Neurosignals 2016;24:25-39.
Drug & Alcohol Dependence
Hypoxia-Inducible Factor-1α Mediates Increased Sympathoexcitation via Glutamatergic N-Methyl-d-Aspartate Receptors in the Paraventricular Nucleus of Rats.
Inhibition of hypoxia-induced cyclooxygenase-2 by Korean Red Ginseng is dependent on peroxisome proliferator-activated receptor gamma  Heewon Song, Young.
by Holly Deng, Hyung Soo Han, Danye Cheng, Guo Hua Sun, and Midori A
Electroacupuncture inhibits excessive interferon-γ evoked up-regulation of P2X4 receptor in spinal microglia in a CCI rat model for neuropathic pain 
Cardioprotective SUR2A promotes stem cell properties of cardiomyocytes
Induction of Neuroendocrine Differentiation in Prostate Cancer Cells by Dovitinib (TKI- 258) and its Therapeutic Implications  Shalini S. Yadav, Jinyi.
Blockade of spinal nerves inhibits expression of neural growth factor in the myocardium at an early stage of acute myocardial infarction in rats  W. Yue,
Volume 68, Issue 2, Pages (August 2005)
H. Wang, Y. Dong, J. Zhang, Z. Xu, G. Wang, C. A. Swain, Y. Zhang, Z
Methadone is a local anaesthetic-like inhibitor of neuronal Na+ channels and blocks excitability of mouse peripheral nerves  C. Stoetzer, K. Kistner,
International Journal of Cardiology
Volume 12, Issue 12, Pages (September 2015)
Volume 80, Issue 6, Pages (September 2011)
Reciprocal regulation by hypoxia-inducible factor-2α and the NAMPT-NAD+-SIRT axis in articular chondrocytes is involved in osteoarthritis  H. Oh, J.-S.
Increased mTORC1 activity contributes to atherosclerosis in apolipoprotein E knockout mice and in vascular smooth muscle cells  Kun Ling Ma, Jing Liu,
Volume 13, Issue 4, Pages (April 2006)
Chronic intrathecal infusion of mibefradil, ethosuximide and nickel attenuates nerve ligation-induced pain in rats  Y.L. Chen, M.L. Tsaur, S.W. Wang,
Volume 130, Issue 6, Pages (May 2006)
15-deoxy-Δ12,14-PGJ2 promotes inflammation and apoptosis in cardiomyocytes via the DP2/MAPK/TNFα axis  Chintan N. Koyani, Werner Windischhofer, Christine.
X. Luo, H. Zhao, L. Hennah, J. Ning, J. Liu, H. Tu, D. Ma 
Α-MSH inhibits TNF-α-induced matrix metalloproteinase-13 expression by modulating p38 kinase and nuclear factor κB signaling in human chondrosarcoma HTB-94.
Morphine tolerance increases [3H]MK-801 binding affinity and constitutive neuronal nitric oxide synthase expression in rat spinal cord  Chih-Shung Wong,
Increased Lipocalin-2 Contributes to the Pathogenesis of Psoriasis by Modulating Neutrophil Chemotaxis and Cytokine Secretion  Shuai Shao, Tianyu Cao,
Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids  H.J. Tu, K.H. Kang, S.Y. Ho, H.C. Liou,
L. Tong, M. Cai, Y. Huang, H. Zhang, B. Su, Z. Li, H. Dong 
Estrogen Upregulates Slug to Enhance the Migration of Keratinocytes
Volume 19, Issue 10, Pages (October 2011)
Unique Interweaved Microtubule Scaffold Mediates Osmosensory Transduction via Physical Interaction with TRPV1  Masha Prager-Khoutorsky, Arkady Khoutorsky,
Mechanosensitive transient receptor potential vanilloid type 1 channels contribute to vascular remodeling of rat fistula veins  Yih-Sharng Chen, MD, PhD,
Tetsuro Tsujimoto, Hiroshi Kajio  International Journal of Cardiology 
Volume 15, Issue 1, Pages (January 2012)
Role of Connective Tissue Growth Factor in Oval Cell Response During Liver Regeneration After 2-AAF/PHx in Rats  Liya Pi, Seh-Hoon Oh, Thomas Shupe, Bryon.
Generating Late-Onset Human iPSC-Based Disease Models by Inducing Neuronal Age- Related Phenotypes through Telomerase Manipulation  Elsa Vera, Nazario.
Development and Dynamic Regulation of Mitochondrial Network in Human Midbrain Dopaminergic Neurons Differentiated from iPSCs  Du Fang, Yu Qing, Shijun.
Marinus A. Borgdorff, Beatrijs Bartelds, Michael G
Volume 22, Issue 11, Pages (November 2015)
Cold temperature induces mucin hypersecretion from normal human bronchial epithelial cells in vitro through a transient receptor potential melastatin.
Renoprotective role of the vitamin D receptor in diabetic nephropathy
KCa1.1 β4-subunits are not responsible for iberiotoxin-resistance in baroreceptor neurons in adult male rats  Wen-Xiao Xu, Tao Ban, Lu-Qi Wang, Miao Zhao,
Shelley Gorman, Melinda A. Judge, Prue H. Hart 
Kellie J. White, Vincent J. Maffei, Marvin Newton-West, Robert A
Spatial and Temporal Recruitment of Androgen Receptor and Its Coactivators Involves Chromosomal Looping and Polymerase Tracking  Qianben Wang, Jason S.
Ex vivo gene delivery of ephrin-B2 induces development of functional collateral vessels in a rabbit model of hind limb ischemia  Masatake Katsu, MD, Hiroyuki.
Volume 65, Issue 1, Pages (January 2004)
Angiotensin-(1–7) inhibits angiotensin II-stimulated phosphorylation of MAP kinases in proximal tubular cells  Z. Su, J. Zimpelmann, K.D. Burns  Kidney.
Retinoid-Induced Epidermal Hyperplasia Is Mediated by Epidermal Growth Factor Receptor Activation Via Specific Induction of its Ligands Heparin-Binding.
STAT proteins mediate angiotensin II–induced production of TIMP-1 in human proximal tubular epithelial cells  Xiangmei Chen, Jianzhong Wang, Feng Zhou,
Volume 79, Issue 4, Pages (February 2011)
Volume 14, Issue 11, Pages (March 2016)
Volume 17, Issue 12, Pages (December 2016)
Chronic AT2 receptor activation increases renal ACE2 activity, attenuates AT1 receptor function and blood pressure in obese Zucker rats  Quaisar Ali,
Volume 129, Issue 2, Pages (April 2007)
Development and Dynamic Regulation of Mitochondrial Network in Human Midbrain Dopaminergic Neurons Differentiated from iPSCs  Du Fang, Yu Qing, Shijun.
Regulation of human renin gene promoter activity: A new negative regulatory region determines the responsiveness to TNFα  Ling-Sing K. Chen, Michael P.
Alleviation of neuropathic pain by intrathecal injection of antisense oligonucleotides to p65 subunit of NF-κB  T. Sun, W.-G. Song, Z.-J. Fu, Z.-H. Liu,
Assessment of pulse transit time to indicate cardiovascular changes during obstetric spinal anaesthesia†  G Sharwood-Smith, J Bruce, G Drummond  British.
Fang Du, Qing Yu, Allen Chen, Doris Chen, Shirley ShiDu Yan 
Volume 3, Issue 6, Pages (December 2008)
Fig. 2 hTERT induces expression of heat shock protein genes through HSF1 and interacts with Hsp70-1. hTERT induces expression of heat shock protein genes.
Presentation transcript:

Angiotensin type 2 receptors in the intermediolateral cell column of the spinal cord: Negative regulation of sympathetic nerve activity and blood pressure  Jie Chao, Juan Gao, Karma-Jaya K. Parbhu, Lie Gao  International Journal of Cardiology  Volume 168, Issue 4, Pages 4046-4055 (October 2013) DOI: 10.1016/j.ijcard.2013.06.051 Copyright © 2013 The Authors Terms and Conditions

Fig. 1 Expression of AT1R and AT2R in spinal cord. A) Confocal immunofluorescent images showing AT1R and AT2R expressions in the T11 segment of the spinal cord. B) Western-blot showing AT1R and AT2R protein expressions in different regions of the spinal cord. (a) Representative blots and (b) mean data of blot density. #P<0.05 vs white matter, *P<0.05 vs IML; n=3 in each group. C) Gene expression of AT1R and AT2R in different regions of the spinal cord. All genes were examined by real-time RT-PCR with gene-specific primers. ΔC(t) (a) and relative fold change to IML (b) were calculated with CT value (see details in Methods). n=3 in each group. International Journal of Cardiology 2013 168, 4046-4055DOI: (10.1016/j.ijcard.2013.06.051) Copyright © 2013 The Authors Terms and Conditions

Fig. 2 Effect of Ang II on sympathetic nerve activity and blood pressure in the IML. A) Representative original recording of MAP and RSNA showing the responses to Ang II (a), Ang II following TEMPO (b), and Ang II following apocynin (c) in the IML. B) Mean data showing change of mean arterial pressure (a), sympathetic nerve activity (b), systolic pressure (c), diastolic pressure (d) and heart rate (e) after treatment with saline, Ang II, Ang II following TEMPO, or Ang II following apocynin, respectively. *P<0.05 vs saline group, #P<0.05 vs Ang II-treated group; n=5 in each group. International Journal of Cardiology 2013 168, 4046-4055DOI: (10.1016/j.ijcard.2013.06.051) Copyright © 2013 The Authors Terms and Conditions

Fig. 3 Effect of AT2R on sympathetic nerve activity and blood pressure. A) Representative original recording of MAP and RSNA showing the responses induced by CGP42112 (a), CGP42112 following PD123319 (b), and CGP42112 following l-NAME (c) in the IML. B) Mean data showing change of mean arterial pressure (a), sympathetic nerve activity (b), systolic pressure (c), diastolic pressure (d) and heart rate (e) after treatment of saline, CGP42112, CGP42112 following PD123319, or CGP42112 following l-NAME, respectively. *P<0.05 vs saline group, #P<0.05 vs Ang II-treated group; n=5 in each group. International Journal of Cardiology 2013 168, 4046-4055DOI: (10.1016/j.ijcard.2013.06.051) Copyright © 2013 The Authors Terms and Conditions

Fig. 4 AT2R activation inhibits the response to AT1R activation. A) Representative original recording of MAP and RSNA showing the responses induced by PD123319 (a), Ang II (b), and Ang II following PD123319 (c) in the IML. B) Mean data showing change of mean arterial pressure (a), sympathetic nerve activity (b), systolic pressure (c), diastolic pressure (d) and heart rate (e) after treatment of saline, PD123319, Ang II or Ang II following PD123319, respectively. *P<0.05 vs saline group, #P<0.05 vs Ang II-treated group; n=5 in each group. International Journal of Cardiology 2013 168, 4046-4055DOI: (10.1016/j.ijcard.2013.06.051) Copyright © 2013 The Authors Terms and Conditions

Fig. 5 Expression of AT1R and AT2R in acute isolated SPNs. Immunostaining of AT1R (A) or AT2R (B) is colocalized with MAP2 and ChAT-positive staining. AT1R and AT2R are colocalized in these neurons (C). DAPI is a nuclear marker; MAP2 is a mature neuronal marker; ChAT is the specific SPN marker. Scale bars, 20μm. International Journal of Cardiology 2013 168, 4046-4055DOI: (10.1016/j.ijcard.2013.06.051) Copyright © 2013 The Authors Terms and Conditions

Fig. 6 Effects of Ang II on electrophysiological characteristics of isolated IML neurons. A) Representative whole cell patch-clamp recording showing that the transient K+ current was enhanced by CGP42112 treatment (100 nM). B) Peak K+ current measured in response to a test pulse from −80 to +80mV. C) Complete current–voltage relationships from vehicle, CGP42112, PD123319, and PD123319+CGP42112 treatments. D) Resting membrane potential. Values are means±SE. *P<0.05 vs vehicle group. #P<0.05 vs CGP42112 group. n=10 in each group. International Journal of Cardiology 2013 168, 4046-4055DOI: (10.1016/j.ijcard.2013.06.051) Copyright © 2013 The Authors Terms and Conditions