Improvement of RNA purification from infected tissues to explore the in vivo host– pathogen interactions with microarrays  Q. Leroy, C. Rovery, D. Raoult,

Slides:



Advertisements
Similar presentations
Genomic analysis of three African strains of Bacillus anthracis demonstrates that they are part of the clonal expansion of an exclusively pathogenic bacterium.
Advertisements

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages
Laboratory diagnosis and biosafety issues of biological warfare agents
C.-S. Lee, J.-H. Lee  Clinical Microbiology and Infection 
P.-Y. Lévy  Clinical Microbiology and Infection 
Gut bacterial microbiota and obesity
Approach to diagnosis of infective endocarditis
S. Wagenpfeil, A. Neiss, K. Banz, P. Wutzler 
Herpes zoster in non-hospitalized children
Migrant health—a cause for concern?
C.-S. Lee, J.-H. Lee  Clinical Microbiology and Infection 
J.-P. Van geertruyden  Clinical Microbiology and Infection 
The microarray technology: facts and controversies
G. Zhao, H. Zhai, Q. Yuan, S. Sun, T. Liu, L. Xie 
R. Cantón  Clinical Microbiology and Infection 
A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU  P.G. Higgins, H. Wisplinghoff, O. Krut, H.
P. Nordmann, L. Poirel  Clinical Microbiology and Infection 
P. Balraj, G. Vestris, D. Raoult, P. Renesto 
Evaluation of differential gene expression in susceptible and resistant clinical isolates of Klebsiella pneumoniae by DNA microarray analysis  A. Doménech-Sánchez,
Louis Pasteur, from crystals of life to vaccination
How to evaluate and predict the ecologic impact of antibiotics: the pharmaceutical industry view from research and development  R. Bax  Clinical Microbiology.
Non-antibiotic strategies for sepsis
Surveillance of Legionnaires’ disease in Austria
S. Richard, P. Seng, P. Parola, D. Raoult, B. Davoust, P. Brouqui 
Vector control: a cornerstone in the malaria elimination campaign
Molecular mechanism of gentamicin resistance in Bartonella henselae
Training for the infectious diseases speciality in Norway
P. Seng, J.M. Rolain, D. Raoult, P. Brouqui 
Does it make sense to detect Streptococcus pyogenes during tonsillitis in Europe to prevent acute rheumatic fever?  S. Edouard, A. Michel-Lepage, D. Raoult 
Laboratory diagnosis and biosafety issues of biological warfare agents
CMI editorial report 2011 Clinical Microbiology and Infection
Elements of design: the knowledge on which we build
Levofloxacin in the treatment of ventilator-associated pneumonia
G. Pappas  Clinical Microbiology and Infection 
W. Saisongkorh, L. Barrassi, B. Davoust, C. -A. de Broucker, D
Metagenomics and probiotics
Laboratory diagnosis of Clostridium difficile disease
A case of pleurisy associated with antibodies to Rickettsia conorii
Rickettsia felis and Rickettsia massiliae in Ivory Coast, Africa
A. Bryskier  Clinical Microbiology and Infection 
The practice of travel medicine in Europe
Nosocomial listeria gastroenteritis in a newborn, confirmed by random amplification of polymorphic DNA  H. Hof, R. Lampidis, J. Bensch  Clinical Microbiology.
Community-acquired pneumonia: epidemiologic and clinical consideration
Abstracts cont. Clinical Microbiology and Infection
Pandemic lineages of extraintestinal pathogenic Escherichia coli
P.M. Hawkey  Clinical Microbiology and Infection 
C. Socolovschi, I. Bitam, D. Raoult, P. Parola 
R. Slinger, I. Moldovan, N. Barrowman, F. Chan 
A.P. Underwood, J. Green  Clinical Microbiology and Infection 
Antimicrobial drug development – the past, the present, and the future
Statin use and clinical outcomes among pneumonia patients
Clinical Microbiology and Infection
Clinical Microbiology and Infection
K. Kaier, N.T. Mutters, U. Frank  Clinical Microbiology and Infection 
Z. Erjavec, H. Kluin-Nelemans, P.E. Verweij 
The atypical pneumonias: clinical diagnosis and importance
Facilitating learning and change in physicians: Implications for a system of continuing medical education in Europe  Robert D. Fox  Clinical Microbiology.
Abstracts Clinical Microbiology and Infection
Modelling during an emergency: the 2009 H1N1 influenza pandemic
S. Shpynov, N. Rudakov, Y. Tohkov, A. Matushchenko, I. Tarasevich, D
J.L. Balcázar  Clinical Microbiology and Infection 
G.C. Schito  Clinical Microbiology and Infection 
Three years experience of real-time PCR for the diagnosis of Q fever
Typing of Clostridium difficile
Impact of antibiotic restrictions: the patient's perspective
Genotyping Rickettsia conorii detected in patients with Mediterranean spotted fever in Algeria using multispacer typing (MST)  L.I. Wenjun, N. Mouffok,
C. Socolovschi, D. Raoult, P. Parola 
Comparative study of pediculicidal effect of medical plants
CMI readers' survey Clinical Microbiology and Infection
The future of diagnostic bacteriology
Presentation transcript:

Improvement of RNA purification from infected tissues to explore the in vivo host– pathogen interactions with microarrays  Q. Leroy, C. Rovery, D. Raoult, P. Renesto  Clinical Microbiology and Infection  Volume 15, Pages 114-115 (December 2009) DOI: 10.1111/j.1469-0691.2008.02189.x Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Transcriptomic profiles of host-pathogens. (a) Electrophoregrams of RNA. (b) RT-PCR amplification using primers targeting ß-actin or rrs. T (total RNA), Rco (purified R. conorii), H (human RNA recovered from beads). (c) M-A plot of biological replicates of R. conorii and human microarrays. Clinical Microbiology and Infection 2009 15, 114-115DOI: (10.1111/j.1469-0691.2008.02189.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions