Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array Eva-Maria King, PhD, Lisa D. Vailes,

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Presentation transcript:

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array Eva-Maria King, PhD, Lisa D. Vailes, MS, Amy Tsay, MSc, Shama M. Satinover, MS, Martin D. Chapman, PhD Journal of Allergy and Clinical Immunology Volume 120, Issue 5, Pages 1126-1131 (November 2007) DOI: 10.1016/j.jaci.2007.06.043 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, Optimization of coupling concentration of IgE capture mAb for detection of total IgE. B, Comparison of total serum IgE levels measured by ImmunoCAP and multiplex; n = 63; ∗∗P < .001. MFI, Median fluorescent intensity. Journal of Allergy and Clinical Immunology 2007 120, 1126-1131DOI: (10.1016/j.jaci.2007.06.043) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Specific IgE responses to indoor and outdoor allergens. Comparison between ELISA and multiplex, n = 95. ∗P < .01. Journal of Allergy and Clinical Immunology 2007 120, 1126-1131DOI: (10.1016/j.jaci.2007.06.043) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 A-D, Comparison of dust mite-specific IgE levels measured by different detection methods: multiplex, D. pteronyssinus (d1 CAP) and streptavidin ImmunoCAP, n = 106. ∗P < .01. Journal of Allergy and Clinical Immunology 2007 120, 1126-1131DOI: (10.1016/j.jaci.2007.06.043) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions