The β-tubulin C-terminal tail modulates the sensitivity of microtubules to paclitaxel. The β-tubulin C-terminal tail modulates the sensitivity of microtubules.

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The β-tubulin C-terminal tail modulates the sensitivity of microtubules to paclitaxel. The β-tubulin C-terminal tail modulates the sensitivity of microtubules to paclitaxel. (A) Microtubule assembly parameters in gene-edited NCI-H460 cells expressing either the full-length βIII-tubulin protein (ZB3), truncated βIII-tubulin protein (ZB3Δ), or βIII-tubulin body with βI-tubulin C-terminal tail (ZB3/CB1) cultured in normal growth media as measured by EB3-mCherry motion and particle tracking in response to paclitaxel treatment (TXL, 6 or 20 nM for 2 h). The microtubule assembly rate (i), microtubule growth length (ii), microtubule growth duration (iii), and number of microtubule growth events (iv) are presented as the mean ± SEM of at least 50 cells in each of three independent experiments for each tubulin modification. *P < 0.05, ***P < 0.001, and ****P < 0.0001 relative to cells expressing the full-length protein (ZB3); #P < 0.05 relative to untreated cells expressing the same type of tubulin modification. (B) Microtubule assembly dynamics as measured by spatiotemporal image correlation spectroscopy for cells treated with paclitaxel (20 nM for 1 or 2 h), showing the speed of cross-correlated movement between the microtubule and EB3 channels (i) and the proportion of microtubule assembly events that are cross-correlated with the microtubule movement (ii). Graphs give the median, box gives the 25th to the 75th percentile, and whiskers give the minimum and maximum values of at least 15 cells from two independent experiments. *P < 0.05,**P < 0.01, ***P < 0.001, and ****P < 0.0001 relative to cells expressing the full-length βIII-tubulin protein; #P < 0.05 relative to untreated cells expressing the same type of tubulin modification. Measurements of untreated cells are reproduced from Fig 3. Corresponding values are presented in Table S1. Amelia L Parker et al. LSA 2018;1:e201800059 © 2018 Parker et al.