A new approach for the separation of spermatozoa from other cell types in forensically relevant samples  F. Grosjean, V. Castella  Forensic Science International:

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A new approach for the separation of spermatozoa from other cell types in forensically relevant samples  F. Grosjean, V. Castella  Forensic Science International: Genetics Supplement Series  Volume 5, Pages e653-e655 (December 2015) DOI: 10.1016/j.fsigss.2015.10.013 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 (A) CD52 stained recovered spermatozoa show a strong shift in fluorescence compared to unstained autofluorescence (grey curve). (B) No shift in fluorescence is observed for recovered epithelial cells compared to autofluorescence (grey curve). (C) Non-separated samples show haploid (n), diploid (2n) peaks and PI negative events. (D) Non-sperm fractions show a diploid PI peak and absence of haploid signal, while sperm fractions (E) show a haploid PI peak. (F) Single male (sperm fraction) and female (non-sperm fraction) DNA profiles were obtained post separation. Boxes indicate amelogenin. Forensic Science International: Genetics Supplement Series 2015 5, e653-e655DOI: (10.1016/j.fsigss.2015.10.013) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

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