FcɛRI cross-linking and IL-3 protect human basophils from intrinsic apoptotic stress Lionel Rohner, MSc, Ramona Reinhart, PhD, Björn Hagmann, PhD, Andrea.

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FcɛRI cross-linking and IL-3 protect human basophils from intrinsic apoptotic stress Lionel Rohner, MSc, Ramona Reinhart, PhD, Björn Hagmann, PhD, Andrea Odermatt, BMA, HF, Annet Babirye, MSc, Thomas Kaufmann, PhD, Michaela Fux, PhD Journal of Allergy and Clinical Immunology Volume 142, Issue 5, Pages 1647-1650.e3 (November 2018) DOI: 10.1016/j.jaci.2018.06.040 Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Survival of resting basophils depends greatly on endogenous Bcl-2, whereas IL-3–activated cells rely on Mcl-1 and Bcl-xL. For technical details, see the Methods section in this article's Online Repository. A and E, Percentages of cells positive for Annexin V, PI, or both are shown. B and D, Fold change of median fluorescence intensity (MFI; Fig 1, B) and ΔMFI (stained minus unstained cells; Fig 1, D) normalized to medium alone (Nil) is shown. C, Representative Western blotting and quantified X-linked inhibitor of apoptosis (XIAP) bands using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for normalization. *P < .05, **P < .01, ***P < .001, and ****P < .0001. n.s., Not significant. Journal of Allergy and Clinical Immunology 2018 142, 1647-1650.e3DOI: (10.1016/j.jaci.2018.06.040) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 FcɛRI receptor cross-linking renders human basophils less susceptible to intrinsic apoptosis through IL-3–dependent and independent signaling. For technical details, see the Methods section in this article's Online Repository. A-C, Percentage of cell death (Fig 2, A) and difference in cell viability of basophils treated with or without ABT compounds compared with their respective FcɛRI cross-linking treatment groups (Fig 2, B and C) or the fraction of basophils negative for both cell death markers (absolute cell number multiplied by survival in percentage). Basophil viability was assessed by using Annexin V/PI (Fig 2, A-C) or Hoechst 33342/PI staining (D). E, Shown is the Δ median fluorescence intensity (stained minus unstained cells) of Bcl-2, Bcl-xL, and Mcl-1 normalized against medium alone (Nil). *P < .05, **P < .01, and ***P < .001. n.s., Not significant. Journal of Allergy and Clinical Immunology 2018 142, 1647-1650.e3DOI: (10.1016/j.jaci.2018.06.040) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 ABT-199 and ABT-263 rapidly trigger caspase-dependent apoptosis in human basophils and eosinophils. A, Basophils, eosinophils, and neutrophils were cultured in medium alone (Nil) or with ABT-199, ABT-263 (300 nmol/L each), or a corresponding concentration of dimethyl sulfoxide (DMSO; 0.003%) in the presence or absence of IL-3 or GM-CSF (10 ng/mL each) for the indicated time periods. STAT, Signal transducer and activator of transcription. B and C, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control and for normalization of immunoreactive bands. Quantified results relative to Nil of caspase-3 activity (Fig E1, B) and Mcl-1 protein level (Fig E1, C) of indicated cell types are presented. Data are shown as mean ± SD fold change over nontreated cells. Untreated cells (Nil) were used as a control sample for the Dunnett post hoc test. *P < .05, **P < .01, and ***P < .001. n.s., Not significant. Journal of Allergy and Clinical Immunology 2018 142, 1647-1650.e3DOI: (10.1016/j.jaci.2018.06.040) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 The Bcl-2 inhibitor ABT-199 and the Bcl-2 and Bcl-xL inhibitor ABT-263 mediate potent and rapid induction of cell death in human basophils and eosinophils. Basophils and eosinophils (A, B, and D) and neutrophils (C) were cultured for 1 to 10 hours (Fig E2, B and C), 24 hours (Fig E2, A), or 72 hours (Fig E2, D) in medium alone (Nil) or in the presence of ABT-199 or ABT-263 used at indicated concentrations (Fig E2, A) or at 300 nmol/L each (Fig E2, B-D). IL-3 was used at 10 ng/mL (Fig E2, D). Percentages of cells that were single and/or double positive for Annexin V and PI are depicted. Untreated cells after 24 hours (Nil; Fig E2, A) or untreated cells after corresponding time points (Nil; Fig E2, B and C) served as a control sample for the Bonferroni multiple comparison posttest for ordinary 1-way ANOVA. *P < .05, **P < .01, ***P < .001, and ****P < .0001. n.s., Not significant. Journal of Allergy and Clinical Immunology 2018 142, 1647-1650.e3DOI: (10.1016/j.jaci.2018.06.040) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions