Stuart I. Myers, MD, Li Wang, BS, Fang Liu, BS, Lori L. Bartula, BS

Slides:

Advertisements

Similar presentations

Volume 59, Issue 3, Pages (March 2001)

Advertisements

Loss of renal function and microvascular blood flow after suprarenal aortic clamping and reperfusion (SPACR) above the superior mesenteric artery is greatly.

Regarding “A 10-year institutional experience with open branched graft reconstruction of aortic aneurysms in connective tissue disorders versus degenerative.

Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells Wen-Jane Lee, PhD, Hsiu-Chung.

The impact of selective visceral perfusion on intestinal macrohemodynamics and microhemodynamics in a porcine model of thoracic aortic cross-clamping

Cyril H. Barton, Zehnmin Ni, Nosratola D. Vaziri Kidney International

Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells Wen-Jane Lee, PhD, Hsiu-Chung.

Hypercholesterolemia and chronic ischemia alter myocardial responses to selective cyclooxygenase-2 inhibition Louis M. Chu, MD, Michael P. Robich, MD,

Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries Jens W Fischer,

Retrograde migration of an abdominal aortic aneurysm endograft leading to postoperative renal failure Barry T. Katzen, MD, Alexandra A. MacLean, MD,

Volume 59, Issue 3, Pages (March 2001)

Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide.

Repetitive progressive thermal preconditioning hinders thrombosis by reinforcing phosphatidylinositol 3-kinase/Akt-dependent heat-shock protein/endothelial.

High-mobility-group box protein 1 A box reduces development of sodium laurate- induced thromboangiitis obliterans in rats Xiangqian Kong, MD, Hai Yuan,

Volume 64, Issue 1, Pages (July 2003)

Volume 64, Issue 5, Pages (November 2003)

Volume 63, Issue 3, Pages (March 2003)

Selective renal blood perfusion induces renal tubules injury in a porcine model Johannes Kalder, MD, Maria Kokozidou, DVM, MSc, PhD, Paula Keschenau,

John C. Kerr, B. S. , Krishna M. Jain, M. D. , Kenneth G. Swan, M. D

Cerebral metabolic recovery from deep hypothermic circulatory arrest after treatment with arginine and nitro-arginine methyl ester Takeshi Hiramatsu,

Iodinated contrast induced renal vasoconstriction is due in part to the downregulation of renal cortical and medullary nitric oxide synthesis Stuart.

Estrogen receptor-mediated enhancement of venous relaxation in female rat: Implications in sex-related differences in varicose veins Joseph D. Raffetto,

Randomized comparison of cold blood and cold crystalloid renal perfusion for renal protection during thoracoabdominal aortic aneurysm repair Scott A.

Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide.

Renal perfusion with venous blood extends the permissible suprarenal clamp time in abdominal aortic surgery Maximilian Pichlmaier, MA, MD, Ludwig Hoy,

Glomerular filtration rate is superior to serum creatinine for prediction of mortality after thoracoabdominal aortic surgery Tam T.T. Huynh, MD, Randolph.

Johannes Kalder, MD, Dieudonne Ajah, Paula Keschenau, MD, Lieven N

Overexpression of transforming growth factor–β1 correlates with increased synthesis of nitric oxide synthase in varicose veins Theresa Jacob, PhD, Anil.

Ambient pressure upregulates nitric oxide synthase in a phosphorylated-extracellular regulated kinase– and protein kinase C–dependent manner Angela G.

Vikas Chander, PhD, Kanwaljit Chopra, PhD Journal of Vascular Surgery

Grace J. Wang, MD, Ronald M. Fairman, MD, Benjamin M

Trimetazidine reduces early and long-term effects of experimental renal warm ischemia: A dose effect study Jerome Cau, MD, Frederic Favreau, PhD, Jean.

Protecting the brain and spinal cord

Volume 79, Issue 1, Pages (January 2011)

Volume 64, Issue 5, Pages (November 2003)

Infected aneurysm of the thoracic aorta

Protection of the kidney after temporary ischemia: Free radical scavengers Kenneth Ouriel, M.D., Nicholas G. Smedira, M.D. *, John J. Ricotta, M.D. Journal.

Superior mesenteric artery outcomes after fenestrated endovascular aortic aneurysm repair Salim Lala, MD, Martyn Knowles, MD, David Timaran, MD, Mirza.

Volume 75, Issue 2, Pages (January 2009)

Shadi J. Abu-Halimah Journal of Vascular Surgery

Cardiopulmonary bypass, myocardial management, and support techniques Changes in autonomic response of the cerebral circulation after normothermic extracorporeal.

Laparoscopic-assisted treatment of abdominal aortic aneurysm requiring suprarenal cross-clamping Mauro Ferrari, MD, Daniele Adami, MD, Raffaella Berchiolli,

Laurent Chiche, MD, Edouard Kieffer, MD Journal of Vascular Surgery

Depressed cardiovascular function and altered platelet kinetics following protamine sulfate reversal of heparin activity Thomas W. Wakefield, M.D., Walter.

Volume 72, Issue 8, Pages (October 2007)

Cyclosporine A attenuates the natriuretic action of loop diuretics by inhibition of renal COX-2 expression Klaus Höcherl, Frieder Kees, Bernhard K. Krämer,

Importance of endothelial nitric oxide synthase for the hypothermic protection of lungs against ischemia-reperfusion injury Li Zhang, MD, Sanjeev Kumar,

Endothelium-dependent contraction of canine coronary artery is enhanced by crystalloid cardioplegic solution Pyng Jing Lin, MD, Chau-Hsiung Chang, MD,

Psoas abscess in patients with an infected aortic aneurysm

Current treatment of renal artery aneurysms may be too aggressive

Inhibition of Glycogen Synthase Kinase-3β Improves Tolerance to Ischemia in Hypertrophied Hearts Rodrigo Barillas, MD, MBA, Ingeborg Friehs, MD, Hung.

Volume 56, Issue 5, Pages (November 1999)

Thrombotic mesenteric ischemia due to aortic dissection

Michel Lacombe, MD Journal of Vascular Surgery

Complement c5a receptor antagonist attenuates multiple organ injury in a model of ruptured abdominal aortic aneurysm Denis W Harkin, MD,, Alex Romaschin,

Journal of Vascular Surgery

Overexpression of endothelial nitric oxide synthase increases skeletal muscle blood flow and oxygenation in severe rat hind limb ischemia Lucy S Brevetti,

Loss of renal function and microvascular blood flow after suprarenal aortic clamping and reperfusion (SPACR) above the superior mesenteric artery is greatly.

Exogenous nitric oxide modulates the systemic inflammatory response and improves kidney function after risk-situation abdominal aortic surgery Francisco.

Iodinated contrast induced renal vasoconstriction is due in part to the downregulation of renal cortical and medullary nitric oxide synthesis Stuart.

Interleukin-1β inhibits PDGF-BB−induced migration by cooperating with PDGF-BB to induce cyclooxygenase-2 expression in baboon aortic smooth muscle cells

Suprarenal aortic clamping and reperfusion decreases medullary and cortical blood flow by decreased endogenous renal nitric oxide and PGE2 synthesis

Outcome after celiac artery coverage during endovascular thoracic aortic aneurysm repair: Preliminary results Sarat K. Vaddineni, MD, Steve M. Taylor,

Chronic digitalis administration alters mesenteric vascular reactivity

Resveratrol neuroprotective effects during focal cerebral ischemia injury via nitric oxide mechanism in rats Shen-Kou Tsai, MD, PhD, Li-Man Hung, PhD,

Nutcracker syndrome due to left-sided inferior vena cava compression and treated with superior mesenteric artery transposition Bao-Zhong Yang, MD, Zhen.

Comparison of supramesenteric aortic cross-clamping with supraceliac aortic cross- clamping for aortic reconstruction Sungho Lim, MD, Pegge M. Halandras,

Uei Pua, MBBS, MMed, FRCR Journal of Vascular Surgery

Prospective randomized study evaluating an absorbable cyanoacrylate for use in vascular reconstructions Alan B. Lumsden, MD, Eugene R. Heyman, PhD Journal.

Brant W. Ullery, MD, George K. Lee, MD, Jason T. Lee, MD

Presentation transcript:

Oxygen-radical regulation of renal blood flow following suprarenal aortic clamping Stuart I. Myers, MD, Li Wang, BS, Fang Liu, BS, Lori L. Bartula, BS Journal of Vascular Surgery Volume 43, Issue 3, Pages 577-586 (March 2006) DOI: 10.1016/j.jvs.2005.10.051 Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 1 The effect of supra-renal aortic clamping above the superior mesenteric artery and reperfusion on blood pressure (A) and heart rate (B). The arterial blood pressure and heart rate were continuously monitored at basal time period and after 30 minutes followed by 60 minutes of reperfusion and compared with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8), with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Data are reported as mm Hg (A) and beats per minute (B) and are expressed as mean ± SEM; a indicates significance at P < .05 compared with sham and c indicates significance at P < .05 compared with L-NAME group. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 2 The effect of supra-renal aortic clamping above the superior mesenteric artery (SMA) and reperfusion on renal cortical blood flow. Laser Doppler probes were placed 2 mm into the cortex, and blood flow was continuously monitored at basal time period and after 30 minutes of supra-renal aortic clamping above the SMA followed by 60 minutes of reperfusion and compared with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8) with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Data are reported as percent change from basal time zero and expressed as mean ± SEM; a indicates significance at P <. 05 compared with sham, b indicates significance at P < .05 compared with the IR group without drug treatment, c indicates significance at P < .05 compared with the L-NAME group, and d indicates significance compared with the I/R + L-arginine group at P < .05. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 3 The effect of supra-renal aortic clamping above the superior mesenteric artery (SMA) and reperfusion on renal medullary blood flow. Laser Doppler probes were placed 4 mm into the medulla, and blood flow was continuously monitored at basal time period and after 30 minutes of supra-renal aortic clamping above the SMA followed by 60 minutes of reperfusion and compared with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8) with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Data are reported as percent change from basal time zero and expressed as mean ± SEM; a indicates significance at P < .05 compared with Sham, b indicates significance at P < .05 compared with the I/R group without drug treatment, c indicates significance at P < .05 compared with the L-NAME group, and d indicates significance compared with the I/R + L-arginine group at P < .05. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 4 The effect of supra-renal aortic clamping above the superior mesenteric artery (SMA) and reperfusion on renal cortical nitric oxide release. Microdialysis probes were placed 2 mm into the cortex, connected to a syringe pump, and perfused in vivo at 3 μL/min with lactated Ringer’s solution. Dialysate fluid was collected at basal time zero and after 30 minutes of supra-renal aortic clamping above the SMA followed by 60 minutes of reperfusion and compared with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8) with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Data are reported as percent change from basal time zero and expressed as mean ± SEM; a indicates significance at P < .05 compared with sham, b indicates significance at P < .05 compared with the I/R group without drug treatment, c indicates significance at P < .05 compared with the L-NAME group, and d indicates significance compared with the I/R + L-arginine group at P < .05. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 5 The effect of supra-renal aortic clamping above the superior mesenteric artery (SMA) and reperfusion on renal medullary nitric oxide release. Microdialysis probes were placed 4 mm into the medulla, connected to a syringe pump, and perfused in vivo at 3 μL/min with lactated ringer’s solution. Dialysate fluid was collected at basal time zero and after 30 minutes of supra-renal aortic clamping above the SMA followed by 60 minutes of reperfusion and compared with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8) with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Data are reported as percent change from basal time zero and expressed as mean ± SEM; a indicates significance at P < .05 compared with sham, b indicates significance at P < .05 compared with the I/R group without drug treatment, c indicates significance at P < .05 compared with the L-NAME group, and d indicates significance compared with the I/R + L-Arginine group at P < .05. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 6 The effect of supra-renal aortic clamping above the superior mesenteric artery and reperfusion on renal cortical (A) and medullary (B) prostaglandin E2 (PGE2) release. Microdialysis probes were placed 2 mm into the cortex, connected to a syringe pump, and perfused in vivo at 3 μL/min with lactated Ringer’s solution. Dialysate fluid was collected at basal time zero and after 30 minutes of supra-renal aortic clamping above the SMA followed by 60 minutes of reperfusion and compared with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8) with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Data are reported as percent change from basal time zero and expressed as mean ± SEM; a indicates significance at P < .05 compared with sham, b indicates significance at P < .05 compared with the I/R group without drug treatment, c indicates significance at P < .05 compared with the L-NAME group, and d indicates significance compared with the I/R + L-Arginine group at P < .05. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 7 The effect of supra-renal aortic clamping above the superior mesenteric artery (SMA) and reperfusion on renal cortical (A) and medullary (B) prostaglandin I2 (PGI2) release. Microdialysis probes were placed 4 mm into the medulla, connected to a syringe pump, and perfused in vivo at 3 μL/min with lactated Ringer’s solution. Dialysate fluid was collected at basal time zero and after 30 minutes of suprarenal aortic clamping above the SMA followed by 60 minutes of reperfusion and compared with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8) with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Data are reported as percent change from basal time zero and expressed as mean ± SEM. PGI2 was measured as the stable metabolite 6-keto-PGF1α. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 8 The effect of supra-renal aortic clamping above the superior mesenteric artery (SMA) and reperfusion on renal cortical (A) and medullary (B) thromboxane B2 (TxB2) release. Microdialysis probes were placed 4 mm into the medulla, connected to a syringe pump, and perfused in vivo at 3 μL/min with lactated Ringer’s solution. Dialysate fluid was collected at basal time zero and after 30 minutes of supra-renal aortic clamping above the SMA followed by 60 minutes of reperfusion and compared with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8) with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Thromboxane A2 measured as the stable metabolite thromboxane B2 (TxB2); a indicates significance at P < .05 compared with sham, and d indicates significance compared with the I/R + L-arginine group at P < .05. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 9 The effect of supra-renal aortic clamping above the superior mesenteric artery and reperfusion (SMA-SPACR) on renal cortical and medullary inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) content. Protein from renal cortex and medulla was prepared from sham and SMA-SRACR animals and subjected to Western blot analysis to identify content of iNOS and COX-2. The blots were analyzed by densitometry. Data are expressed as densitometry units ± SEM (n = 8); a indicates significance at P < .05 compared with sham. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 10 The effect of supra-renal aortic clamping above the superior mesenteric artery and reperfusion (SMA-SRACR) on renal cortical and medullary prostaglandin E2 synthase (E2) and prostacyclin synthase (PS) content. Protein from renal cortex and medulla was prepared from sham and SMA-SRACR animals and subjected to Western blot analysis to identify E2 and PS content. The blots were analyzed by densitometry. Data are expressed as densitometry units ± SEM (n = 8). Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 11 The effect of supra-renal aortic clamping above the superior mesenteric artery (SMA) and reperfusion on creatinine clearance (CrCl). Serum and urine were collected from separate groups of animals to measure serum creatinine, urine creatinine, and urine volume to calculate creatinine clearance. The serum and urine were collected at basal time zero and after 30 minutes of supra-renal aortic clamping above the SMA followed by 60 minutes of reperfusion and compared to with sham operation. The ischemia/reperfusion (I/R) groups were treated with saline carrier (I/R group, n = 10), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME) (30 mg/kg, nitric oxide synthase inhibitor, n = 8), or L-arginine (400 mg/kg, nitric oxide precursor, n = 8) with and without superoxide dismutase (SOD) (10,000 U/kg, oxygen-derived free radical scavenger, n = 8). Data are reported as mL/min (mean ± SEM; a indicates significance at P < .05 compared with sham, b indicates significance at P < .05 compared with the I/R group without drug treatment, c indicates significance at P < .05 compared with the L-NAME group, and d indicates significance compared with the I/R + L-arginine group at P < .05. Journal of Vascular Surgery 2006 43, 577-586DOI: (10.1016/j.jvs.2005.10.051) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions