Masato Mizuashi, Tomoyuki Ohtani, Satoshi Nakagawa, Setsuya Aiba

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Redox Imbalance Induced by Contact Sensitizers Triggers the Maturation of Dendritic Cells Masato Mizuashi, Tomoyuki Ohtani, Satoshi Nakagawa, Setsuya Aiba Journal of Investigative Dermatology Volume 124, Issue 3, Pages 579-586 (March 2005) DOI: 10.1111/j.0022-202X.2005.23624.x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Concentration of simple chemicals to induce cell death of monocyte-derived dendritic cells (MoDC). MoDC were treated with various concentrations of contact sensitizers or non-sensitizers for 24 h. Then the total percentage of both apoptotic cells (annexin V (+)) and necrotic cells (propidium iodide (PI) (+)) was assessed by flow cytometry. The mean±SEM of the percentage of annexin V-positive cells or PI-positive cells from seven independent experiments is shown. Asterisks indicate significance (**p<0.01, *p<0.05) for the difference between stimulated cells and non-treated cells. Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Some contact sensitizers at sublethal concentrations upregulated the expression of CD86 in monocyte-derived dendritic cells (MoDC). MoDC were either unstimulated or stimulated with sublethal concentrations of contact sensitizers, NiCl2, MnCl2, 2,4-dinitrochlorobenzene (DNCB), HCHO, and thimerosal, or non-sensitizers, ZnCl2, SDS, and benzalkonium chloride (BC) for 48 h. The surface expression of CD86 antigen was analyzed by flow cytometry. The mean±SEM of the relative mean fluorescence intensities (MFI) was determined from four to six independent experiments. Asterisks indicate significance (**p<0.01, *p<0.05) for the difference between stimulated cells and relevant control cells, non-treated, or those treated with dimethyl sulfoxide (DMSO). Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 2,4-dinitrochlorobenzene (DNCB) and NiCl2 reduced the reduced/oxidized glutathione (GSH/GSSG) ratio in monocyte-derived dendritic cells (MoDC) at different kinetics. MoDC were cultured with 300 μM or 1 mM of NiCl2 or 10 or 30 μM of DNCB for 0, 1, 3, 10, 30, 60, and 120 min. After stimulation with the chemicals, the GSH/GSSG ratio in MoDC was assessed using colorimetric assays by the GSH reductase-DTNB recycling procedure. The relative GSH/GSSG ratio was calculated by the following formula. The relative GSH/GSSG=the GSH/GSSG ratio in MoDC treated with chemicals/that in non-treated MoDC. The mean±SEM of the relative GSH/GSSG ratio from three independent experiments is shown. Asterisks indicate significance (**p<0.01, *p<0.05) for the difference between stimulated cells and the non-treated control. Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 All contact sensitizers, but not non-sensitizers, at sublethal concentrations decreased the reduced/oxidized glutathione (GSH/GSSG) ratio in monocyte-derived dendritic cells (MoDC). MoDC were either unstimulated or stimulated with three concentrations of contact sensitizers, NiCl2, MnCl2, 2,4-dinitrochlorobenzene (DNCB), HCHO, and thimerosal, or non-sensitizers, ZnCl2, SDS, and benzalkonium chloride (BC) for 2 h. The three concentrations of each chemical consisted of a lethal concentration, sublethal concentration, and 1/3 of the sublethal concentration. Intracellular GSH and GSSG were measured using colorimetric assays by the GSH reductase-DTNB recycling procedure. The relative GSH/GSSG ratio was calculated as described in Materials and Methods. Each dot corresponds to the ratio obtained from different experiments and the mean±SEM of the relative GSH/GSSG ratio is also presented. Asterisks indicate significance (**p<0.01, *p<0.05) for the difference between stimulated cells and the non-treated control. Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Contact sensitizers phosphorylated p38 mitogen-activated protein kinase (MAPK) in monocyte-derived dendritic cells (MoDC). MoDC were either unstimulated or stimulated with sublethal or lethal concentrations of contact sensitizers, NiCl2, 2,4-dinitrochlorobenzene (DNCB) HCHO, thimerosal, or non-sensitizer, benzalkonium chloride (BC), or sodium dodecyl sulfate (SDS) for different time periods, and the phosphorylation of p38 MAPK was analyzed by flow cytometry. Representative flow cytometry from three independent experiments 60 min after chemical stimulation is shown in (a). The time course of phosphorylation of p38 MAPK after stimulation of NiCl2 or DNCB and that after stimulation of HCHO, thimerosal, BC, or SDS is shown in (b) and (c), respectively. Asterisks indicate significance (**p<0.01, *p<0.05) for the difference between stimulated cells and the non-treated control. Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 N-acetyl-L-cysteine (NAC) increased the reduced/oxidized glutathione (GSH/GSSG) ratio in monocyte-derived dendritic cells (MoDC) with or without chemical stimulation. To examine the effects of the antioxidant NAC, we added 25 mM NAC to the culture of MoDC 30 min prior to stimulation by the chemicals. Two hours after culture, the relative GSH/GSSG ratio of MoDC was calculated as described in Materials and Methods. The mean±SEM of the relative GSH/GSSG ratio from three to seven independent experiments is shown. Asterisks indicate significance (**p<0.01, *p<0.05) for the difference between MoDC treated with NAC and those untreated with NAC. Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 N-acetyl-L-cysteine (NAC) suppressed the phosphorylation of p38 mitogen-activated protein kinase (MAPK) in monocyte-derived dendritic cells (MoDC) stimulated with the contact sensitizers. MoDC were pretreated with 25 mM NAC for 30 min and then cultured with 300 μM or 1 mM of NiCl2 (a) or 10 or 30 μM of 2,4-dinitrochlorobenzene (DNCB) (b). After 0–60 min of culture, the phosphorylation of p38 MAPK was analyzed by flow cytometry. The relative GSH/GSSG ratio was calculated as described in Materials and Methods. This is a representative flow cytometry of two independent experiments. Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 8 N-acetyl-L-cysteine (NAC) suppressed the augmentation of CD86 expression in monocyte-derived dendritic cells (MoDC) stimulated with the contact sensitizers. MoDC were pretreated with 25 mM NAC for 30 min and then cultured with or without 300 μM of NiCl2, 10 μM of 2,4-dinitrochlorobenzene (DNCB), 1 × 10-3% benzalkonium chloride (BC), or 0.01% dimethyl sulfoxide (DMSO). After 2 d of culture, their surface expression of CD86 was analyzed by flow cytometry. The histogram of chemical-treated MoDC in the absence or the presence of 25 mM NAC is expressed as a thick solid line or a solid line, whereas a shaded line or a dotted line indicates the histogram of non-treated dendritic cell (DC) or that of DC stained with an isotype control antibody, respectively. This is a representative flow cytometry of three independent experiments. Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 9 Most contact sensitizers, but not non-sensitizers, at sublethal concentrations lowered the reduced/oxidized glutathione (GSH/GSSG) ratio in human monocytic cell-lineTHP-1 cells, and the effect that was abrogated by N-acetyl-L-cysteine (NAC). Human monocytic cell-line THP-1 cells were either unstimulated or stimulated with three concentrations of contact sensitizers, NiCl2, 2,4-dinitrochlorobenzene (DNCB), HCHO, and thimerosal, or non-sensitizers, SDS and benzalkonium chloride (BC) for 2 h, and then, the GSH/GSSG ratio was measured by the same procedure as shown in Figure 4. At sublethal concentrations, all contact sensitizers except for HCHO, but none of the non-sensitizers, lowered the GSH/GSSG ratio in THP-1 cells. The means±SEM of the GSH/GSSG ratio was determined in five independent experiments. Asterisks indicate significance (**p<0.01, *p<0.05) for the difference between stimulated cells and the non-treated control. Journal of Investigative Dermatology 2005 124, 579-586DOI: (10.1111/j.0022-202X.2005.23624.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions