Human Somatic Cell Nuclear Transfer Using Adult Cells

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Presentation transcript:

Human Somatic Cell Nuclear Transfer Using Adult Cells Young Gie Chung, Jin Hee Eum, Jeoung Eun Lee, Sung Han Shim, Vicken Sepilian, Seung Wook Hong, Yumie Lee, Nathan R. Treff, Young Ho Choi, Erin A. Kimbrel, Ralph E. Dittman, Robert Lanza, Dong Ryul Lee Cell Stem Cell Volume 14, Issue 6, Pages 777-780 (June 2014) DOI: 10.1016/j.stem.2014.03.015 Copyright © 2014 Elsevier Inc. Terms and Conditions

Cell Stem Cell 2014 14, 777-780DOI: (10.1016/j.stem.2014.03.015) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 1 Pluripotency, Cytogenetic, and Genetic Analysis of Human SCNT ESC Lines (A) SCNT-derived hatching blastocysts and their ESC colonies. Both SCNT ESC lines expressed standard stem cell markers: OCT-4, SSEA-4, TRA-1-60, and TRA-1-81. Bars indicate 100 μ. (B) Cytogenetic G-banding analysis showed that CHA-NT2 and CHA-NT4 had normal male karyotypes (46, XY). (C) Nuclear DNA genotyping using 16 STR markers (15 on autosomal loci and 1 on X/Y locus), and (D) Mitochondrial DNA genotyping on three single nucleotide polymorphic (SNP) sites. m.8794C > T (rs2298007), m.10398A > G (rs2853826), and m.8697G > A (rs28358886) demonstrate that the nuclear DNAs in both CHA-NT2 and CHA-NT4 were exclusively derived from male DFB cells, and mt-DNA were derived from the oocytes. See also Figures S1 and S2. Cell Stem Cell 2014 14, 777-780DOI: (10.1016/j.stem.2014.03.015) Copyright © 2014 Elsevier Inc. Terms and Conditions