Analysis of Tumor Cell Evolution in a Melanoma: Evidence of Mutational and Selective Pressure for Loss of p16ink4 and for Microsatellite Instability

Slides:

Advertisements

Similar presentations

Amy M. Dworkin, Stephanie Y. Tseng, Dawn C. Allain, O

Advertisements

Somatic Mutation of the 5′ Noncoding Region of the BCL-6 Gene Is Associated with Intraclonal Diversity and Clonal Selection in Histological Transformation.

Genotyping of Chimerical BCR-ABL1 RNA in Chronic Myeloid Leukemia by Integrated DNA Chip Jong-Hun Kang, Hyun-Gyung Goh, Sang-Ho Chae, Sung-Yong Kim,

A Rapid Polymerase Chain Reaction-Based Screening Method for Identification of All Expanded Alleles of the Fragile X (FMR1) Gene in Newborn and High-Risk.

Development and Applications of a BRAF Oligonucleotide Microarray

Molecular Diagnosis in Ewing Family Tumors

Jeung-Yeal Ahn, Katie Seo, Olga Weinberg, Scott D. Boyd, Daniel A

Thomas W. Prior, Scott J. Bridgeman

Marguerite Stas, Hugo Degreef Journal of Investigative Dermatology

Liisa Väkevä, Annamari Ranki Journal of Investigative Dermatology

Active Influx Transport is Mediated by Members of the Organic Anion Transporting Polypeptide Family in Human Epidermal Keratinocytes Ruth Schiffer, Mark.

Expression of Two Breast-Specific Molecules in the Lung

Molecular Aspects of Melanocytic Dysplastic Nevi

Screening for Expanded Alleles of the FMR1 Gene in Blood Spots from Newborn Males in a Spanish Population Isabel Fernandez-Carvajal, Paulina Walichiewicz,

Kavitha K. Reddy Journal of Investigative Dermatology

Microsatellite Instability in Benign Skin Lesions in Hereditary Non-Polyposis Colorectal Cancer Syndrome Victoria J. Swale, Anthony G. Quinn, Veronique.

High Frequency of Loss of Heterozygosity on Chromosome Region 9p21–p22 but Lack of p16INK4a/p19ARF Mutations in Greek Patients with Basal Cell Carcinoma.

Activation of a Cryptic Splice Site of PTEN and Loss of Heterozygosity in Benign Skin Lesions in Cowden Disease Stephen J. Meltzer, Manfred Wolter Journal.

Suzanne M. Morgan, Elizabeth Hodges, Tracey J

Laurent Gouya Journal of Investigative Dermatology

Circulating Tumor Cells and Melanoma Progression

Hotspot Mutation of Brahma in Non-Melanoma Skin Cancer

Detection of a Peripheral Blood T Cell Clone is an Independent Prognostic Marker in Mycosis Fungoides Elisabeth A. Fraser-Andrews, Alison J. Woolford,

Andrew Rowan, Ian Tomlinson Journal of Investigative Dermatology

HPV Sequences in Blood of Patients with Condyloma Acuminata

Application of COLD-PCR for Improved Detection of NF2 Mosaic Mutations

P16INK4a and p14ARF Tumor Suppressor Genes Are Commonly Inactivated in Cutaneous Squamous Cell Carcinoma Victoria L. Brown, Catherine A. Harwood, Tim.

Mutational Heterogeneity in Melanoma: An Inconvenient Truth

The Melanocortin 5 Receptor is Expressed in Human Sebaceous Glands and Rat Preputial Cells Diane Thiboutot, Aruntha Sivarajah, Kathryn Gilliland, Zhaoyuan.

Lisa Edelmann, Jianli Dong, Robert J. Desnick, Ruth Kornreich

Vikas K. Goel, Alexander J. F. Lazar, Carla L. Warneke, Mark S

Amplification Refractory Mutation System, a Highly Sensitive and Simple Polymerase Chain Reaction Assay, for the Detection of JAK2 V617F Mutation in Chronic.

Clinical Snippets Journal of Investigative Dermatology

Minutes of the Board of Directors Meeting

Amplification and Overexpression of the EMS 1 Oncogene, a Possible Prognostic Marker, in Human Hepatocellular Carcinoma Bao-Zhu Yuan, Xiaoling Zhou,

Novel Polymorphism in the FMR1 Gene Resulting in a “Pseudodeletion” of FMR1 in a Commonly Used Fragile X Assay Thomas M. Daly, Arash Rafii, Rick A. Martin,

Somatic Mutations of Fas (Apo-1/CD95) Gene in Cutaneous Squamous Cell Carcinoma Arising from a Burn Scar Sug Hyung Lee, Min Sun Shin, Hong Sug Kim, Won.

Journal of Investigative Dermatology

Journal of Investigative Dermatology

Carol S. Trempus, John E. French, Raymond W. Tennant

S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A

MiRNA Expression Profiling in Melanocytes and Melanoma Cell Lines Reveals miRNAs Associated with Formation and Progression of Malignant Melanoma Daniel.

Society for Investigative Dermatology 2010 Meeting Minutes

Isoform-Selective Upregulation of Mast Cell Chymase in the Development of Skin Fibrosis in Scleroderma Model Mice Eiichi Kakizoe, Naotaka Shiota, Yoko.

BRAF and NRAS Mutations Are Frequent in Nodular Melanoma but Are not Associated with Tumor Cell Proliferation or Patient Survival Lars A. Akslen, Sabrina.

Research Snippets Journal of Investigative Dermatology

Gabriella Esposito, Giuseppe Rescigno, Francesco Salvatore

Fetal Microchimerism Is Common in Normal and Diseased Vulvar Skin

Wook Lew Journal of Investigative Dermatology

Katie Ridd, Siegrid Yu, Boris C. Bastian

Research Snippets from the British Journal of Dermatology

Circulating Tumor Cells and Detection of the Melanoma-Associated Antigen HMW-MAA in the Serum of Melanoma Patients Anja Ulmer, Gerhard Fierlbeck Journal.

Expression of Opsin Molecule in Cultured Murine Melanocyte

Promoter Hypermethylation of the O6-Methylguanine DNA Methyltransferase Gene and Microsatellite Instability in Metastatic Melanoma Maija R.J. Kohonen-Corish,

Allelic Loss at Drosophila Patched Gene Is Highly Prevalent in Basal and Squamous Cell Carcinomas of the Skin Hadi Danaee, Margaret R. Karagas, Karl.

Research Snippets Journal of Investigative Dermatology

Journal of Investigative Dermatology

Xiangfeng Cui, Helen Feiner, Honghua Li

Detection of BRCA1 and BRCA2 Ashkenazi Jewish Founder Mutations in Formalin- Fixed Paraffin-Embedded Tissues Using Conventional PCR and Heteroduplex/Amplicon.

BRAF Mutations Are Common Somatic Events in Melanocytic Nevi1

CDKN2A and MC1R Mutations in Patients with Sporadic Multiple Primary Melanoma Ketty Peris, Maria Concetta Fargnoli, Alessia Pacifico, Tiziana Surrenti,

Consequences of Psychological Distress in Adolescents with Acne

Journal of Investigative Dermatology

Activation of B-raf in Non-Malignant Nevi Predicts a Novel Tumor Suppressor Gene in Melanoma (MAP Kinase Phosphatase) Jack L. Arbiser Journal of Investigative.

Method of Mutation Analysis May Contribute to Discrepancies in Reports of V599EBRAF Mutation Frequencies in Melanocytic Neoplasms Christopher J. Miller,

Journal of Investigative Dermatology

Maureen J. O'Sullivan, Peter A. Humphrey, Louis P. Dehner, John D

An Association Between Sebaceous Carcinoma and Microsatellite Instability in Immunosuppressed Organ Transplant Recipients Catherine A. Harwood, Victoria.

Comparison of Genetic Profiles Between Primary Melanomas and their Metastases Reveals Genetic Alterations and Clonal Evolution During Progression Reiji.

K. Miura, M. Obama, K. Yun, H. Masuzaki, Y. Ikeda, S. Yoshimura, T

Presentation transcript:

Analysis of Tumor Cell Evolution in a Melanoma: Evidence of Mutational and Selective Pressure for Loss of p16ink4 and for Microsatellite Instability Albert Rübben, Philipp Babilas, Jens M. Baron, Anja Hofheinz, Mark Neis, Florian Sels, Markus Sporkert Journal of Investigative Dermatology Volume 114, Issue 1, Pages 14-20 (January 2000) DOI: 10.1046/j.1523-1747.2000.00838.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Competitive multiplex PCR for demonstration of loss of the p16ink4 gene. (a) Calibration of primer ratio, (b) PCR with extracted DNA from primary tumor (P1–P3), metastases (M6, M7) and blood of the patient. pBR322, molecular size marker. Journal of Investigative Dermatology 2000 114, 14-20DOI: (10.1046/j.1523-1747.2000.00838.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Comparison of the methods used for the analysis of PCR-amplified microsatellite DNA. (a) TG-SSCP analysis, (b) analysis in a sequencing gel, (c) conventional sequencing gel versus Southern blot hybridization of the blotted sequencing gel with a (CA)10-oligonucleotide; M, metastases; N, normal formalin-fixed tissue specimens; ▸ loss of DNA bands (LOH); ▹ mutated DNA bands (microsatellite instability); ◃* points to nonspecific DNA bands. Journal of Investigative Dermatology 2000 114, 14-20DOI: (10.1046/j.1523-1747.2000.00838.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Characterization of tumor cell populations. TG-SSCP analysis of PCR-amplified microsatellite DNA obtained from microdissected tissue of the primary tumor (P1–3), from metastases (M7, M6) and from the patient’s blood (B). ▸ loss of DNA bands (LOH); ▹ mutated DNA bands. Journal of Investigative Dermatology 2000 114, 14-20DOI: (10.1046/j.1523-1747.2000.00838.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Putative genealogic tree of tumor cell evolution in the analysed patient obtained from data summarized in Table 1. Journal of Investigative Dermatology 2000 114, 14-20DOI: (10.1046/j.1523-1747.2000.00838.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions