BET and MEK inhibition synergistically impair cell cycle regulation and activate apoptotic signaling BET and MEK inhibition synergistically impair cell.

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BET and MEK inhibition synergistically impair cell cycle regulation and activate apoptotic signaling BET and MEK inhibition synergistically impair cell cycle regulation and activate apoptotic signaling Venn diagram of genes that were up‐ or downregulated after treatment of NRAS‐mutant M93‐047 cells with 0.5 μM JQ‐1, 0.1 μM PD901, or combination (JQ‐1/PD901) for 48 h (P < 0.05, FDR < 5%).Ingenuity pathway analysis of functions significantly deregulated (P < 0.05) by combination treatment with JQ‐1/PD901 for 48 h.Heat map representation of the common cluster of differentially expressed genes (n = 1,129 genes) from 12 samples treated with DMSO, JQ‐1, PD901, or combination for 48 h.RPPA was performed in M93‐047 and WM3000 cells treated with BETi (JQ‐1 or OTX) or MEKi PD901 for 48 h as single agents or in combination. Analysis was performed in three biological replicates.Immunoblotting in a panel of NRAS‐mutant melanoma cells (M93‐047, WM3000, and WM852) treated with vehicle, JQ‐1, PD901, or combination for 48 h. Source data are available online for this figure. Ileabett M Echevarría‐Vargas et al. EMBO Mol Med. 2018;10:e8446 © as stated in the article, figure or figure legend