Comparison of cytokine profiles induced by ChiLob 7/4 and TLR ligands in ex vivo whole-blood stimulation assays. Comparison of cytokine profiles induced.

Slides:

Advertisements

Similar presentations

Glioma-derived soluble factor(s) enhance IL-10 signaling.

Advertisements

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M

Volume 143, Issue 2, Pages (February 2013)

Characterization of a rare IL-10–competent B-cell subset in humans that parallels mouse regulatory B10 cells by Yohei Iwata, Takashi Matsushita, Mayuka.

Involvement of suppressors of cytokine signaling in toll-like receptor–mediated block of dendritic cell differentiation by Holger Bartz, Nicole M. Avalos,

IL-1β significantly augments the angiogenic activity of NSCLC by inducing the expression of angiogenic CXC chemokine genes. IL-1β significantly augments.

Melanoma donor (MD) NK cells are functionally impaired/exhausted.

Impaired Toll-like receptor 8–mediated IL-6 and TNF-α production in antigen-presenting cells from patients with X-linked agammaglobulinemia by Klára Sochorová,

Indoleamine 2,3-dioxygenase specific, cytotoxic T cells as immune regulators by Rikke Bæk Sørensen, Sine Reker Hadrup, Inge Marie Svane, Mads Christian.

Ex vivo effects of proNGF and NGF on JIA mononuclear cells.

Heating is a multifunctional adjuvant that affects tumor microenvironment through several intrinsic and extrinsic mechanisms, which could enhance immunotherapy.

Cytokine and chemokine expression of flucloxacillin-specific T cell clones (TCC) of patient P1. Cytokine and chemokine expression of flucloxacillin-specific.

2008: A MyD88 O, DC Bali Pulendran, Weiping Cao Immunity

Adult chronic idiopathic thrombocytopenic purpura (ITP) is the manifestation of a type-1 polarized immune response by Fotios P. Panitsas, Maria Theodoropoulou,

Enhancement of the host immune responses in cutaneous T-cell lymphoma by CpG oligodeoxynucleotides and IL-15 by Maria Wysocka, Bernice M. Benoit, Sarah.

Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes. Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes.

Tumor necrosis factor-α production in whole blood after cardiopulmonary bypass: Downregulation caused by circulating cytokine-inhibitory activities J.

Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide F.G. Araujo, T.L. Slifer, J.S. Remington Clinical.

The PI3K–Akt pathway is essential for the in vivo maintenance of murine Tregs. The PI3K–Akt pathway is essential for the in vivo maintenance of murine.

Pg and Ec LPS induced levels of soluble mediators from in cultures of individuals with and without T2D with periodontitis. Pg and Ec LPS induced levels.

Maturation, Ag presentation capacity, and IL-12 production of DCs

The absence of ADCC by nivolumab in vitro.

Figure 5 Cytokine release and stimulation of cells during alemtuzumab treatment Cytokine release and stimulation of cells during alemtuzumab treatment.

Distinct phases in recovery of reconstituted innate cellular-mediated immunity after murine syngeneic bone marrow transplantation Jeffery J. Auletta,

IL32 prompts cell activation and cancer-related pathways.

Ex vivo IL-6 production. Ex vivo IL-6 production. Human whole blood was stimulated with the indicated doses of the E. coli O113 endotoxin that was used.

Unstimulated levels of soluble mediators from in cultures of individuals with and without T2D with periodontitis. Unstimulated levels of soluble mediators.

WNT ligands contribute to the immune response during septic shock and amplify endotoxemia-driven inflammation in mice by Marcela Gatica-Andrades, Dimitrios.

(A–C) Time-course changes in morning time fasted, and daytime (A) IL-6, (B) TNF-α, and (C) β-hydroxybutyrate concentrations. (A–C) Time-course changes.

Volume 138, Issue 1, Pages (January 2010)

Profiles of anti-inflammatory cytokines after endotoxin injection.

Effects of E. coli and S. aureus infection on levels of TNF-α and IL-1β in milk. Effects of E. coli and S. aureus infection on levels of TNF-α and IL-1β.

by Defne Bayik, Debra Tross, Lydia A

Role of TLR signaling in hY4-induced changes and effects of TLR inhibition. Role of TLR signaling in hY4-induced changes and effects of TLR inhibition.

Figure 5 ADP-induced inflammatory responses require P2Y12 receptors in human microgliaIncreasing concentrations of ADP (5, 50, and 200 μM) increased tumor.

Arp2/3-mediated formation of nuclear actin networks is essential for CD4+ T cell effector functions. Arp2/3-mediated formation of nuclear actin networks.

Effect of CIA and treatment with 3,4-dichloropropionaniline (DCPA) on production of key serum inflammatory factors. Effect of CIA and treatment with 3,4-dichloropropionaniline.

Intestinal myofibroblasts in innate immune responses of the intestine

IFNα Induces MDSC Maturation and Loss of Suppressive Function

CD4+CLA+CD103+T cells from human blood and skin share a functional profile. CD4+CLA+CD103+T cells from human blood and skin share a functional profile.

Cytokine changes in LPS-induced TNFα and spontaneous IL-6 production in whole-blood cultures. Cytokine changes in LPS-induced TNFα and spontaneous IL-6.

Treatment effects of fenretinide (4-HPR, 5 μmol/L), 2-ME (2

The presence of soluble IL12p70 during moDCpoly stimulation of KIRnegNKG2Aneg cells results in a dominant population of multifunctional NKG2Apos NK cells.

coTCRcys-transduced T cells control tumor growth in vivo.

IL6 mRNA is not detected in metastatic prostate cancer cells.

Cytokine expression in the ileum and colon.

pDCs from the melanoma environment responded to TLR-L stimulation.

PD-1 inhibition stimulates the proliferation and cytokine secretion of exhausted/senescent CD8+ T cells in vitro. PD-1 inhibition stimulates the proliferation.

Comparison of OPN production by mature DCs and activated macrophages.

Effect of TLR-4 ligation with LPS on NF-κB activation in EOC cells: (A) MyD88+ and (B) MyD88− EOC cells were treated with 10 μg/mL LPS for 1, 2, and 4.

Distributions of CD4, Th1, and Th2 in adults (n = 16; A) and children (n = 50; B). Distributions of CD4, Th1, and Th2 in adults (n = 16; A) and children.

Supernatants of the positive proliferative responses indicated in Table 1 were analyzed for the presence of IFNγ, tumor necrosis factor-α, IL-2, IL-4,

Effect of anti-cyCD79b (10D10) and anti-cyCD79b (10D10)-MCC-DM1 on cytokine levels in cynomolgus monkeys. Effect of anti-cyCD79b (10D10) and anti-cyCD79b.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

IL8 and TNFα are required for neutrophil-induced UMSCC47 increase in invasion. IL8 and TNFα are required for neutrophil-induced UMSCC47 increase in invasion.

Representative cytokine and cytotoxicity responses to CMV peptides on day 0 and day 7 in one HLA A2+ donor. Representative cytokine and cytotoxicity responses.

In vitro time course assays for four renal selective compounds.

T cell effector cytokines and staphylococcal products regulate CCL18.

PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate distinct mechanisms of regulation. PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate.

Only IFNγ shows consistently increased mRNA expression in mycosis fungoides (MF) lesions. mRNA expression levels of various cytokines in the skin of healthy.

Schematic model illustrating how paracrine signaling drives LPS-stimulated cytokine secretion. Schematic model illustrating how paracrine signaling drives.

Comparison of in vivo activity of 4D5scFvZZ and 4D5scFv.

LPS inhibits polyI:C-induced proinflammatory cytokines in human DCs

Monocytes from melanoma patients are unresponsive to TLR3 agonists.

Combination of R848 and anti-CD200R affects activation of tumor-infiltrating myeloid cells. Combination of R848 and anti-CD200R affects activation of tumor-infiltrating.

T cells with a ROR1-specific CAR eliminate tumor cells in vivo only with a modified long spacer. T cells with a ROR1-specific CAR eliminate tumor cells.

ADU-S100 primes the innate immune system in tolerant, tumor bearing neu/N mice. ADU-S100 primes the innate immune system in tolerant, tumor bearing neu/N.

Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. PCR.

Induction of PD-1 in B cells by TLR agonists and primary HCC-SN.

Effect of aspirin (ASA) on serum cytokine concentration in hemodialysis (HD) patients. Effect of aspirin (ASA) on serum cytokine concentration in hemodialysis.

Presentation transcript:

Comparison of cytokine profiles induced by ChiLob 7/4 and TLR ligands in ex vivo whole-blood stimulation assays. Comparison of cytokine profiles induced by ChiLob 7/4 and TLR ligands in ex vivo whole-blood stimulation assays. LPS was the most potent ligand for stimulating release of cytokines. Soluble ChiLob 7/4 (CL7/4) and CpG were not effective at producing cytokines above unstimulated levels. The cytokine expression profile for cross-linked ChiLob 7/4 was similar to that produced by LPS, producing significantly high concentrations (*, P < 0.05) of MIP1α, MIP1β, TNFα, IL-6, IL-8, and IL-12 compared with unstimulated, although the levels of MIP1α, MIP1β, IL-6, IL-8, and IL-12 produced with cross-linked ChiLob 7/4 were significantly lower than that produced by LPS (**). Low but significant levels of IL-4 and IL-10 above unstimulated were also produced (***). The results show the mean response of 3 donors. F. Chowdhury et al. Cancer Immunol Res 2014;2:229-240 ©2014 by American Association for Cancer Research