Renoprotective immunosuppression by pioglitazone with low-dose cyclosporine in rat heart transplantation Yosuke Tanaka, MD, Tomomi Hasegawa, MD, PhD,

Slides:

Advertisements

Similar presentations

Aprotinin improves kidney function and decreases tubular cell apoptosis and proapoptotic signaling after renal ischemia-reperfusion Ajay Kher, MD, Kirstan.

Advertisements

Max B. Mitchell, MD The Journal of Thoracic and Cardiovascular Surgery

Manuel J. Antunes, MD, PhD, DSc

Transplantation of hypoxia-preconditioned mesenchymal stem cells improves infarcted heart function via enhanced survival of implanted cells and angiogenesis

Simvastatin attenuates cardiac isograft ischemia-reperfusion injury by down-regulating CC chemokine receptor-2 expression Rong Yin, MD, Jiaquan Zhu,

Inhibition of the MEK/ERK pathway reduces microglial activation and interleukin-1-beta expression in spinal cord ischemia/reperfusion injury in rats

The use of technetium tc 99m annexin V for in vivo imaging of apoptosis during cardiac allograft rejection Patrick W. Vriens, MDa, Francis G. Blankenberg,

Annexin V homodimer protects against ischemia reperfusion–induced acute lung injury in lung transplantation Kohei Hashimoto, MD, Hyunhee Kim, MSc, Hisashi.

Prediction of severe acute kidney injury after pediatric cardiac surgery with the use of novel biomarkers: A new trend in clinical research and risk stratification

High basal level of autophagy in high-altitude residents attenuates myocardial ischemia– reperfusion injury Yijie Hu, MD, Qi Sun, MD, Zhiping Li, MD,

Effects of azithromycin and tanomastat on experimental bronchiolitis obliterans Katharina Krenn, MD, Matthias Gmeiner, MD, PhD, Patrick Paulus, MD, Nezir.

Volume 87, Issue 4, Pages (April 2015)

Exogenous surfactant attenuation of ischemia–reperfusion injury in the lung through alteration of inflammatory and apoptotic factors Bart P. van Putte,

Changfa Guo, MD, Husnain Kh. Haider, PhD, Winston S. N

Christopher G. A. McGregor, MB, William R

Free-radical scavenger edaravone inhibits both formation and development of abdominal aortic aneurysm in rats Keisuke Morimoto, MD, Tomomi Hasegawa,

The adverse effect of back-bleeding from lumbar arteries on spinal cord pathophysiology in a rabbit model Yujiro Kawanishi, MD, Kenji Okada, MD, Hiroshi.

Cardiac surgery–associated neutrophil gelatinase–associated lipocalin score for postoperative acute kidney injury: What is the clinical implication?

Pioglitazone preserves vein graft integrity in a rat aortic interposition model Zhi Chen, MD, Tomomi Hasegawa, MD, PhD, Akiko Tanaka, MD, Yutaka Okita,

Building a bioartificial heart: A 3-song saga

Volume 85, Issue 1, Pages (January 2014)

Keratinocyte growth factor accelerates compensatory growth in the remaining lung after trilobectomy in rats Keitaro Matsumoto, MD, Takeshi Nagayasu,

Intrinsic cardiac stem cells are essential for regeneration

Centers for Disease Control “increased-risk” organ donor: Not so risky? Francis D. Pagani, MD, PhD The Journal of Thoracic and Cardiovascular Surgery

Volume 82, Issue 9, Pages (November 2012)

William M. DeCampli, MD, PhD

Philippe Menasché, MD, PhD

Efficacy and safety of inhaled tacrolimus in rat lung transplantation

Cardiac stem cells in the real world

A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure Akiko.

The lord of the rings Antonio Miceli, MD, PhD

Autologous fibrin-coated small-caliber vascular prostheses improve antithrombogenicity by reducing immunologic response Tomomi Hasegawa, MD, PhD, Kenji.

Perioperative cyclooxygenase 2 inhibition to reduce tumor cell adhesion and metastatic potential of circulating tumor cells in non–small cell lung cancer

Direct visualization of the aortic cusp from the left ventricle during aortic root reimplantation Yutaka Okita, PhD, MD, Takanori Oka, MD, Shunsuke Miyahara,

Aldo R. Castañeda, MD, PhD: Recipient of the Lifetime Achievement Award and 74th president of The American Association for Thoracic Surgery Pedro J.

Yang Zhang, MD, Haiquan Chen, MD, PhD

Aditya K. Kaza, MD The Journal of Thoracic and Cardiovascular Surgery

Seeing a tree through the forest: Precision medicine tools can enhance donor allocation in heart transplantation Vishnu Vasanthan, MD, Paul W.M. Fedak,

Elective cardiac retransplantation: A viable option that can be repeated Hannah Copeland, MD, Romana Coelho-Anderson, RN, CCTC, Nicole Mineburg, RN, CCTC,

All that glitters may not be gold: Changes in serum creatinine may not be the criterion standard for acute kidney injury Rakesh C. Arora, MD, PhD, FRCSC,

Pathologic features of cryopreserved aortic allograft implanted in the active infection Akitoshi Yamada, MD, Kenji Okada, MD, Rei Takahashi, MD, Yutaka.

Selective phosphodiesterase-5 inhibition reduces neointimal hyperplasia in rat carotid arteries after surgical endarterectomy Kristóf Hirschberg, MD,

Volume 65, Issue 5, Pages (May 2004)

It's not “just a shunt” but sometimes it should be…

Genetic deficiency of adiponectin protects against acute kidney injury

Military surgeons just want to have fun

A first start for lung transplantation?

Volume 70, Issue 6, Pages (September 2006)

Daniel T. Engelman, MD, FACS, Michael J. Germain, MD

A fate worse than death Jennifer S. Lawton, MD

Video-assisted resection for lung cancer results in fewer complications Lunxu Liu, PhD, MD, FRCS The Journal of Thoracic and Cardiovascular Surgery

Passing the torch The Journal of Thoracic and Cardiovascular Surgery

Is single-dose prophylactic gentamicin associated with acute kidney injury in patients undergoing cardiac surgery? A matched-pair analysis Dorthe Viemose.

The origins of open heart surgery at the University of Minnesota 1951 to 1956 Richard A. DeWall, MD The Journal of Thoracic and Cardiovascular Surgery

The Journal of Thoracic and Cardiovascular Surgery

Discussion The Journal of Thoracic and Cardiovascular Surgery

The future of cardiac surgery training: A survival guide

Surgical outcomes after cardiac surgery in liver transplant recipients

The continuing challenge of congenital heart disease in China

Selective cyclooxygenase 2 inhibitor induces indefinite survival of fully allogeneic cardiac grafts and generates CD4+ regulatory cells Takeshi Yokoyama,

Activation of transglutaminase type 2 for aortic wall protection in a rat abdominal aortic aneurysm formation Takashi Munezane, MD, Tomomi Hasegawa,

“The more things change…”: The challenges ahead

Why arch curvature affects arch resistance

Commentary: Avoiding acute kidney injury after cardiac operations: Searching for the holy grail is not easy Victor A. Ferraris, MD, PhD The Journal.

Elective cardiac retransplantation: A viable option that can be repeated Hannah Copeland, MD, Romana Coelho-Anderson, RN, CCTC, Nicole Mineburg, RN, CCTC,

Did you like Terminator 3 better than Terminator 2

Prolonged mechanical unloading preserves myocardial contractility but impairs relaxation in rat heart of dilated cardiomyopathy accompanied by myocardial.

A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure Akiko.

Akiko Tanaka, MD, PhD, Takeyoshi Ota, MD, PhD

Gene transfection of hepatocyte growth factor attenuates the progression of cardiac remodeling in the hypertrophied heart Keiji Iwata, MD, Yoshiki Sawa,

Presentation transcript:

Renoprotective immunosuppression by pioglitazone with low-dose cyclosporine in rat heart transplantation Yosuke Tanaka, MD, Tomomi Hasegawa, MD, PhD, Zhi Chen, MD, Yutaka Okita, MD, PhD, Kenji Okada, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 138, Issue 3, Pages 744-751 (September 2009) DOI: 10.1016/j.jtcvs.2009.04.019 Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Effects of pioglitazone and cyclosporine on allograft survival in our rat heart transplantation model. ∗P < .005. Pio, Pioglitazone; CsA, cyclosporine A; NS, not significant. Data were analyzed by using a log-rank test of Kaplan–Meier analysis. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 744-751DOI: (10.1016/j.jtcvs.2009.04.019) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Histologic findings of cardiac allografts in acute rejection. A, Hematoxylin and eosin (HE) staining and immunohistochemical staining of CD4 and CD8. Bar = 100 μm. B, Rejection score. C, Quantitative analysis of CD4+ and CD8+ cells. NS, Not significant. All data are expressed as means ± standard errors of the mean. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 744-751DOI: (10.1016/j.jtcvs.2009.04.019) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Serum concentrations of interleukin 2 (IL-2; A) and interferon γ (IFN-γ; B) by means of enzyme-linked immunosorbent assay. NS, Not significant. All data are expressed as means ± standard errors of the mean. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 744-751DOI: (10.1016/j.jtcvs.2009.04.019) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Effects of pioglitazone and cyclosporine on recipient renal function: A, serum BUN levels; B, serum creatinine levels; and C, creatinine clearance. NS, Not significant. All data are expressed as means ± standard errors of the mean. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 744-751DOI: (10.1016/j.jtcvs.2009.04.019) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Histologic findings of renal apoptosis after heart transplantation. A, transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining and immunofluorescent staining of Bax. TUNEL-positive cells (brown) are shown by arrows. Intracellular expression of Bax is identified by the green emission. Cell nuclei are identified by the blue emission. Bar = 100 μm. B, Quantitative analysis of TUNEL-positive cells. All data are expressed as means ± standard errors of the mean. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 744-751DOI: (10.1016/j.jtcvs.2009.04.019) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 6 Renal expression of peroxisome proliferator–activated receptor γ (PPAR-γ) and transforming growth factor β1 (TGF-β1) after heart transplantation. A, Immunofluorescent staining of PPAR-γ and TGF-β1. Intracellular expression of PPAR-γ and TGF-β1 is identified by the green emission. Cell nuclei are identified by the blue emission. Bar = 100 μm. B, TGF-β1 protein levels in recipient kidney, as determined by means of enzyme-linked immunosorbent assay. NS, Not significant. All data are expressed as means ± standard errors of the mean. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 744-751DOI: (10.1016/j.jtcvs.2009.04.019) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions