Yeast Transformation Mod 2 Day 2 Thursday Oct 9th.

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Yeast Transformation Mod 2 Day 2 Thursday Oct 9th

A little more about your yeast NY411 MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52, trp1d63

A little more about your yeast NY411 MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52, trp1d63 Ty917

A little more about your yeast NY411 MAT(A) his4-917d, lys2-173R2, leu2d1, ura3-52, trp1d63

Transformation Wash the cells with Wash Sol’n (= water?) Resuspend the cells in Competent Sol’n (= LiAc?) Mix with your DNA samples No DNA, pRS414, concentrated/cleaned PCR Resuspend in Transformation Sol’n (= PEG?) 1 hr, 30° Plate on SC-trp

Select TRP+ transformants

For next time…. Work on your materials and methods to this point Read article for discussion in lab Revise Mod1 report if you want Enjoy the fall weekend…you’ve all been working SO hard