Gene Expression Profiling during All-trans Retinoic Acid-Induced Cell Differentiation of Acute Promyelocytic Leukemia Cells Lijun Yang, Hongshan Zhao,

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Gene Expression Profiling during All-trans Retinoic Acid-Induced Cell Differentiation of Acute Promyelocytic Leukemia Cells Lijun Yang, Hongshan Zhao, Shi-Wu Li, Kim Ahrens, Christine Collins, Sarah Eckenrode, Qing-guo Ruan, Richard A. McIndoe, Jin-Xiong She The Journal of Molecular Diagnostics Volume 5, Issue 4, Pages 212-221 (November 2003) DOI: 10.1016/S1525-1578(10)60476-X Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Expression profiles of 12,288 genes in NB4 cells with or without ATRA (1 μmol/L) treatment. A: Hierarchical clustering of 12,288 genes based on their expression profiles in NB4 cells treated with ATRA at 12 hours, 24 hours, 48 hours, 72 hours, and 96 hours. Each row represents a single gene on the microarray, and each column a separated sample at a different time point. The expression profile of functionally related genes: mitogen activated protein kinase (B), protein kinase C and protein kinase A-related genes (C), IFN-induced genes (D), and neutrophil marker protein genes (E). The Journal of Molecular Diagnostics 2003 5, 212-221DOI: (10.1016/S1525-1578(10)60476-X) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 RT-PCR analysis of genes in NB4 cells treated with ATRA. cDNA was synthesized from equal amounts of RNA isolated from NB4 cells treated with ATRA at 0, 12, 24, 48, 72, and 96 hours separately. The data are representative examples of three independent experiments that gave similar results. Actin serves as internal control. The Journal of Molecular Diagnostics 2003 5, 212-221DOI: (10.1016/S1525-1578(10)60476-X) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Differentiation of ATRA-induced NB4 cells. A: Morphological features of NB4 cells in response to ATRA treatment at different times. B: Nitroblue tetrazolium enzymatic reduction in ATRA-treated NB4 cells. C: CD11b intergrin expression following ATRA treatment of NB4 cells at each time point as assessed by flow cytometric analysis. The Journal of Molecular Diagnostics 2003 5, 212-221DOI: (10.1016/S1525-1578(10)60476-X) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions