Fig. 2 In vitro assessment of hESC-RPE cell sheets.

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Fig. 2 In vitro assessment of hESC-RPE cell sheets. In vitro assessment of hESC-RPE cell sheets. (A) Macroscopic photographic images of a hESC-RPE cell sheet after 6 weeks in culture. The right-hand image shows the hESC-RPE cells on the human amniotic membrane (hAM) scaffold. Scale bar, 50 μm. (B) Section of the hESC-RPE cell sheet illustrating the monolayer organization of hESC-RPE cells. Cells were stained for TYRP1 expression (red) and counterstained with DAPI (blue). The hAM scaffold is indicated. Scale bar, 50 μm. (C) hESC-RPE cells cultured on the hAM scaffold and stained for specific RPE markers. Top: ZO-1, green; MITF, red. Bottom: DAPI, blue; TYRP1, red. Scale bars, 50 μm. (D) Transmission electron microscopy image of the hESC-RPE cell sheet. Scale bar, 5 μm. (E) Scanning electron microscopy images of the hESC-RPE cell sheet showing hESC-RPE cells on the hAM scaffold at different magnifications. The bottom image (a magnification of the area indicated by the rectangle in the top image) shows the basement membrane and extracellular matrix fibers of the hAM scaffold. Scale bars, 5 μm (top) and 1 μm (bottom). Three different hESC-RPE cell batches were tested. Karim Ben M’Barek et al., Sci Transl Med 2017;9:eaai7471 Published by AAAS