Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique Giuseppe Ricci, M.D., Sandra Perticarari, B.Sc.,

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Presentation transcript:

Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique Giuseppe Ricci, M.D., Sandra Perticarari, B.Sc., Rita Boscolo, B.Sc., Marcella Montico, B.Sc., Secondo Guaschino, M.D., Gianni Presani, B.Sc. Fertility and Sterility Volume 91, Issue 2, Pages 632-638 (February 2009) DOI: 10.1016/j.fertnstert.2007.11.068 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Cytofluorimetric analysis of dot-plot with log of green fluorescence (Syto16) versus log of red (7-AAD) fluorescence (A, B, C) displaying viable (R2), apoptotic (R3) and dead (R4) sperm cells. Plots D, E, F represent FSC versus SSC parameters on the basis of which a gate R5 sperm was set up to eliminate cell debris from analysis. Region R6 represents the bead population for absolute count. Cytograms A and D represent the analysis of semen sample before preparation, B and E after density-gradient centrifugation, C and F after swim-up technique. Fertility and Sterility 2009 91, 632-638DOI: (10.1016/j.fertnstert.2007.11.068) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions