Comparison of QIAsymphony Automated and QIAamp Manual DNA Extraction Systems for Measuring Epstein-Barr Virus DNA Load in Whole Blood Using Real-Time.

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Presentation transcript:

Comparison of QIAsymphony Automated and QIAamp Manual DNA Extraction Systems for Measuring Epstein-Barr Virus DNA Load in Whole Blood Using Real-Time PCR Stella Laus, Lawrence A. Kingsley, Michael Green, Robert M. Wadowsky The Journal of Molecular Diagnostics Volume 13, Issue 6, Pages 695-700 (November 2011) DOI: 10.1016/j.jmoldx.2011.07.006 Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 The proportion of positive EBV PCR reactions for blood samples with various EBV DNA load values. Solid symbols, QIAsymphony-extracted samples; open symbols, QIAamp-extracted samples. The proportion values were obtained from extraction of six replicates and testing each in duplicate PCR reactions (n = 12). The Journal of Molecular Diagnostics 2011 13, 695-700DOI: (10.1016/j.jmoldx.2011.07.006) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Logarithmic linear correlation between results obtained with the QIAsymphony and the QIAamp extraction systems for 48 sample pairs with viral loads of ≥1000 copies/mL. Solid circles show the paired results obtained with the two extraction systems, the solid line shows the fit of the linear regression, and the dashed line is the theoretical line of perfect agreement. The Journal of Molecular Diagnostics 2011 13, 695-700DOI: (10.1016/j.jmoldx.2011.07.006) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Comparison of the QIAsymphony and QIAamp extraction systems shown as a Bland-Altman plot. Solid circles show the difference between the two methods for whole blood samples with viral loads of ≥1000 copies/mL (n = 48 pairs), and the short-dash line plots the mean difference value (y = 0.18 log copies/mL) between the two methods. The solid line is the theoretical line of perfect agreement between the two methods (y = 0.0 log copies/mL), and the long-dash lines show the mean difference ± 2 SD. The line of mean difference and the line of perfect agreement are separated to a small but definite extent (0.18 log copies/mL). The Journal of Molecular Diagnostics 2011 13, 695-700DOI: (10.1016/j.jmoldx.2011.07.006) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions