Exogenous IL-4 cannot rescue Th2 cytokine expression in HuR-deficient cells. Exogenous IL-4 cannot rescue Th2 cytokine expression in HuR-deficient cells. (A) YFP+, YFP−, or WT CD4+ T cells were stimulated or not with 100 U/ml rIL-4. Five days postactivation, cells were harvested and restimulated with PMA and ionomycin for 5 h, and cytokine production was assessed by intracellular cytokine staining. (B) p-Stat5 and p-Stat6 levels in YFP+, YFP−, and WT CD4+ T cells activated in the presence of IL-4. (C) CD25 expression in YFP+, YFP−, and WT CD4+ T cells activated in the presence or absence of 1000 U/ml rIL-4 for 5 d. Data (mean + SEM) are a representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with the Tukey multiple-comparisons test. Patsharaporn Techasintana et al. ImmunoHorizons 2017;1:109-123 Copyright © 2017 The Authors