TR specifically inhibits NLRP3 inflammasome activation

Slides:

Advertisements

Similar presentations

CHO et al. Supplementary Figure 1 * * * Supplementary Fig. 1. Primary human epidermal keratinocytes were grown in dermal cell culture medium with growth.

Advertisements

Figure 1 A B * C 100 x 200 x 400 x D GSEControl. Mafbx β-tubulin Control GSE * Figure 2.

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

Volume 20, Issue 4, Pages (July 2017)

BRD4 expression levels are associated with sensitivity to BET inhibition in NRAS‐mutant melanoma BRD4 expression levels are associated with sensitivity.

Lipopolysaccharide Activates Caspase-1 (Interleukin-1–Converting Enzyme) in Cultured Monocytic and Endothelial Cells by Ralf R. Schumann, Claus Belka,

The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth A22Rv1.

PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection Proportion.

Control of IL‐6 synthesis by ζPKC and RelA Ser311 phosphorylation.

[68Ga]Pentixafor PET imaging of MM xenografts Real‐time PCR analysis of cxcr4 transcript expression levels in HeLa (negative control) and in MM cell lines.

Volume 38, Issue 6, Pages (June 2013)

CAR T cell dose and percentages of CAR‐positive cells within CAR T cell products CAR T cell dose and percentages of CAR‐positive cells within CAR T cell.

Fstl1 promotes cardiac fibroblasts proliferation via the ERK1/2‐dependent pathway Fstl1 promotes cardiac fibroblasts proliferation via the ERK1/2‐dependent.

TR suppresses the assembly of NLRP3 inflammasome

Effect of PRK and SRB on Mtb H37Rv survival

Tumour‐induced IL6 increases systemic cholesterol levels

Volume 36, Issue 3, Pages (March 2012)

Volume 35, Issue 6, Pages (December 2011)

Allergy-Inducing Chromium Compounds Trigger Potent Innate Immune Stimulation Via ROS-Dependent Inflammasome Activation Christian Adam, Jonas Wohlfarth,

Volume 45, Issue 4, Pages (October 2016)

KLK7 is involved in the Aβ degradation activity

Expression of PD‐1 on PBMC and malignant exudate T cells

Volume 153, Issue 2, Pages (April 2013)

Effect of VEGFA on ALDH+ population maintenance

Volume 150, Issue 3, Pages (August 2012)

Volume 39, Issue 2, Pages (August 2013)

BRD4 expression levels are associated with sensitivity to BET inhibition in NRAS‐mutant melanoma BRD4 expression levels are associated with sensitivity.

Volume 48, Issue 1, Pages e6 (January 2018)

SEMA3C silencing inhibits CRPC cell growth and induces apoptosis

SPRY2 deficiency‐induced IL6 drives CRPC by elevating tumoral HSD3B1 and cholesterol levels SPRY2 deficiency‐induced IL6 drives CRPC by elevating tumoral.

Volume 36, Issue 3, Pages (March 2012)

Volume 7, Issue 1, Pages (January 2010)

Volume 14, Issue 7, Pages (February 2016)

Volume 12, Issue 1, Pages (July 2015)

LPS-induced caspase-11 processing is independent of the NLRP3 and caspase-1 inflammasome. LPS-induced caspase-11 processing is independent of the NLRP3.

Activation of inflammasomes by EBV‐associated factors and microenvironmental stressors.A.Expression of inflammasome components in NPC cell lines. Activation.

Association of AIM2, RIG‐I and NLRP3 inflammasomes with better survival in NPC patients.A.Overexpression of ASC, caspase‐1 and IL‐1β proteins as well as.

Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+ tumors Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+

Dangerous Liaisons: Mitochondrial DNA Meets the NLRP3 Inflammasome

Akt inhibition with AZD5363 does not enhance the radiosensitivity of tumour cells in vitro Akt inhibition with AZD5363 does not enhance the radiosensitivity.

Volume 46, Issue 3, Pages (March 2017)

TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

The preventive role of TR in HFD‐treated mice

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

CD137/NFATc1 Axis Activated NLRP3 Inflammasome in Vascular Smooth Muscle Cells (A) Western blot and quantitative results of NLRP3, pro-IL-1β, and mature.

High‐fat diet augments gut permeability, metabolic endotoxemia, and systemic inflammation High‐fat diet augments gut permeability, metabolic endotoxemia,

Activation of miR‐21‐3p by PPARβ/δ requires activation of the TGFβ receptor Activation of miR‐21‐3p by PPARβ/δ requires activation of the TGFβ receptor.

Surface biotinylation and fate of TREM2

Volume 34, Issue 2, Pages (February 2011)

The HDAC3 inhibitor suppresses PTEN‐deficient prostate cancer growth

Therapeutic role of TR in HFD‐established diabetic mice

Both E102Q and L95fs Sig1R variants are unstable and lose their abilities to control Ca2+ flux in Neuro2a cells Both E102Q and L95fs Sig1R variants are.

Etifoxine modulation of T‐cell activity during EAE

TR directly binds to NLRP3 and inhibits its oligomerization

Volume 160, Issue 1, Pages (January 2015)

SPRY2 deficiency‐induced IL6 drives CRPC by elevating tumoral HSD3B1 and cholesterol levels SPRY2 deficiency‐induced IL6 drives CRPC by elevating tumoral.

P2X4R blockade increases pro‐inflammatory gene expression after EAE

Nilotinib inhibits DDR1 invasive activity of patient‐derived CRC cells

Human Monocytes Engage an Alternative Inflammasome Pathway

Volume 150, Issue 3, Pages (August 2012)

SEMA3C regulates prostate cancer cell growth

The GBS hemolytic pigment/lipid toxin induces caspase 1 activation and pyroptosis THP‐1 macrophages were treated with GBS pigment, and caspase 1 activation.

Niacin suppresses pro‐inflammatory cytokine expression in macrophages in DSS‐induced colitis in mice Niacin suppresses pro‐inflammatory cytokine expression.

Residual enzymatic activity in media or cell lysates containing 1G244 and titration of 1G244-induced cell death in B6Nlrp1b+ BMDMs. Residual enzymatic.

Volume 25, Issue 9, Pages e5 (November 2018)

Volume 24, Issue 9, Pages e5 (August 2018)

Sean P. Cullen, Conor J. Kearney, Danielle M. Clancy, Seamus J. Martin

Volume 43, Issue 2, Pages (August 2015)

Volume 21, Issue 1, Pages (January 2017)

Presentation transcript:

TR specifically inhibits NLRP3 inflammasome activation TR specifically inhibits NLRP3 inflammasome activation AImmunoblot analysis of IL‐1β and cleaved caspase‐1 (p20) in culture supernatants (SN) of LPS‐primed BMDMs treated with various doses (above lanes) of TR and then stimulated with nigericin, and immunoblot analysis of the precursors of IL‐1β (pro‐IL‐1β) and caspase‐1 (pro‐casp1) in lysates of those cells (Input).B–DELISA of IL‐1β (B), TNF‐α (C) and IL‐6 (D) in supernatants from LPS‐primed BMDMs treated with various doses (above lanes) of TR and then stimulated with nigericin.EImmunoblot analysis of IL‐1β and cleaved caspase‐1 (p20) in supernatants from PMA‐differentiated THP‐1 cells treated with various doses (above lanes) of TR and then stimulated with nigericin.FImmunoblot analysis of IL‐1β and cleaved caspase‐1 (p20) in culture supernatants (SN) of LPS‐primed BMDMs treated with TR (100 μM) and then stimulated with MSU, nigericin, ATP, Alum, and immunoblot analysis of the precursors of IL‐1β (pro‐IL‐1β) and caspase‐1 (pro‐casp1) in lysates of those cells (Input).GELISA of IL‐1β in culture supernatants (SN) of LPS‐primed BMDMs treated with TR (100 μM) and then stimulated with MSU, nigericin, ATP, Alum.HImmunoblot analysis of IL‐1β and cleaved caspase‐1 (p20) in culture supernatants (SN) of Pam3‐primed BMDMs treated with various doses (above lanes) of TR and then stimulated with cytosolic LPS (cLPS), and immunoblot analysis of the precursors of IL‐1β (pro‐IL‐1β) and caspase‐1 (pro‐casp1) in lysates of those cells (Input).IELISA of IL‐1β in culture supernatants (SN) of Pam3‐primed BMDMs treated with various doses (above lanes) of TR and then stimulated with cytosolic LPS (cLPS).Data information: Data are from three independent experiments with biological duplicates in each (B–D, G, I; mean and s.e.m. of n = 6) or are representative of at least three independent experiments (A, E, F, H). Statistics were analyzed using an unpaired Student's t‐test: **P < 0.01, ***P < 0.001.Source data are available online for this figure. Yi Huang et al. EMBO Mol Med. 2018;emmm.201708689 © as stated in the article, figure or figure legend