(A) ChIP-qPCR to verify the recruitment of ISWI to the UAS sites

Slides:

Advertisements

Similar presentations

Gene expression (signal intensity) Control Osmotic Salt Drought Root Control Gene expression (signal intensity) Treatment.

Advertisements

Figure S1 A B The ratio of UMTs/CMTs by averaging every 100 peaks. A. Pol-II data, B. ER data. It showed the ratio is decreasing along the lower level.

Volume 15, Issue 3, Pages (April 2016)

The INO80 Complex Removes H2A

Volume 16, Issue 12, Pages (September 2016)

Roger B. Deal, Steven Henikoff Developmental Cell

Volume 44, Issue 3, Pages (November 2011)

Steven J. Petesch, John T. Lis Cell

Volume 54, Issue 1, Pages (April 2014)

Volume 8, Issue 2, Pages (July 2014)

The ribozyme approach distinguishes RNA-dependent and RNA-independent functions of lincRNA genes. The ribozyme approach distinguishes RNA-dependent and.

by Kentson Lam, Alexander Muselman, Randal Du, Yuka Harada, Amanda G

Loss of ACF1 affects transcription prominently in inactive chromatin.

(A) Schematic representation of the conditional Lats1 locus.

Volume 60, Issue 4, Pages (November 2015)

Genome-wide expression screen identifies novel hypoxia-regulated genes

Volume 50, Issue 6, Pages (June 2013)

Volume 16, Issue 12, Pages (September 2016)

Expression of pathogenesis-related (PR) genes in transgenic barley in response to pathogen inoculation. Expression of pathogenesis-related (PR) genes in.

Volume 7, Issue 6, Pages (June 2014)

Volume 62, Issue 1, Pages (April 2016)

ACF1 represses multiple reporters in a context-dependent manner.

Volume 16, Issue 3, Pages (March 2015)

Volume 19, Issue 12, Pages (June 2017)

Systematic mapping of functional enhancer-promoter connections with CRISPR interference by Charles P. Fulco, Mathias Munschauer, Rockwell Anyoha, Glen.

Volume 17, Issue 12, Pages (December 2016)

(A) Genotyping of the Lats2 and Cre alleles in the PyMT-derived cell lines (see primers location in Fig S2A). (A) Genotyping of the Lats2 and Cre alleles.

Volume 44, Issue 3, Pages (November 2011)

The INO80 Complex Removes H2A

Volume 64, Issue 6, Pages (December 2016)

Validation of ChIP-seq reads.

Volume 25, Issue 13, Pages e5 (December 2018)

Conserved Histone Variant H2A

DNMT3A variants cause unique DNAm signatures.

Systematic mapping of functional enhancer–promoter connections with CRISPR interference by Charles P. Fulco, Mathias Munschauer, Rockwell Anyoha, Glen.

Volume 36, Issue 4, Pages (February 2016)

Panoramix enforces piRNA-dependent cotranscriptional silencing

Fig. 2 Genotype-induced differential gene expression is different in MDMi cells compared to monocytes. Genotype-induced differential gene expression is.

The Activation Potential of MOF Is Constrained for Dosage Compensation

Volume 42, Issue 6, Pages (June 2011)

Volume 61, Issue 3, Pages (February 2016)

Volume 20, Issue 13, Pages (September 2017)

Anne Pfeiffer, Hui Shi, James M. Tepperman, Yu Zhang, Peter H. Quail

Volume 20, Issue 3, Pages (July 2017)

Identification of the uvh6-4 mutation.

MYC regulates the antitumor immune response through CD47 and PD-L1

ACF1 loss perturbs gene expression in early embryos.

(A) Comparison of wild-type and AcfC transcriptomes in relation to the chromatin five-state model. (A) Comparison of wild-type and AcfC transcriptomes.

ACF can be tethered to a reporter locus through GAL4-DBD.

Dnmt2 mutants show delayed immune responses and Dnmt2–EGFP relocalizes and interacts with DCV RNA during infection. Dnmt2 mutants show delayed immune responses.

Transcriptome changes in Cnot6l knockout oocytes and zygotes.

(A) Schematic representation of the conditional Lats2 locus.

CTCF Binding Polarity Determines Chromatin Looping

Fig. 3 Conserved genomic association of PRC1 activity in different leukemic cells. Conserved genomic association of PRC1 activity in different leukemic.

Protection of Germline Gene Expression by the C

Enhancer RNA Facilitates NELF Release from Immediate Early Genes

GATC sites where methylation changes over time correspond to a change in gene expression in a dam mutant. GATC sites where methylation changes over time.

CaQTL analysis identifies genetic variants affecting human islet cis-RE use. caQTL analysis identifies genetic variants affecting human islet cis-RE use.

DRN and DRNL regulate STM expression via binding to a conserved promoter motif. DRN and DRNL regulate STM expression via binding to a conserved promoter.

(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.

Structure of Wt1 genomic regions in human and zebrafish.

mRNA adenosine-to-inosine editing increases under DR.

Enrichment of miRNAs among DPAR genes and their relative expression under dietary restriction. Enrichment of miRNAs among DPAR genes and their relative.

The MEG3 promoter and enhancer interact with other putative enhancers within the DLK1-MEG3–imprinted region. The MEG3 promoter and enhancer interact with.

RA signaling in early postnatal PFC

Volume 17, Issue 12, Pages (December 2016)

Pervasive Targeting of Nascent Transcripts by Hfq

Specific DNA methylation and expression signatures associated with binding of E2A–PBX1 fusion protein. Specific DNA methylation and expression signatures.

The Transcription and H3K4me3 Methylation Levels of Many SAGs Are Upregulated in jmj16-1. The Transcription and H3K4me3 Methylation Levels of Many SAGs.

PIF3 Inhibits the Binding of TCP4 to the Promoters of SAUR Genes and Represses Their Expression. PIF3 Inhibits the Binding of TCP4 to the Promoters of.

Presentation transcript:

(A) ChIP-qPCR to verify the recruitment of ISWI to the UAS sites (A) ChIP-qPCR to verify the recruitment of ISWI to the UAS sites. “UAS” and “5′ LacZ” denote the regions amplified by qPCR. (A) ChIP-qPCR to verify the recruitment of ISWI to the UAS sites. “UAS” and “5′ LacZ” denote the regions amplified by qPCR. Bars denote average % input enrichment (n = 3 biological replicates) ± SEM. “neg ctrl” represents a negative control locus (encompassing the Spt4 gene). (B) RT–qPCR assesses expression changes of the LacZ gene in early embryos (2–8 h) upon ACF1 tethering. Bars denote average fold-change over the control (GAL4DBD alone) (n = 3 biological replicates) ± SEM. (C) Screenshot of the genome browser representing the LacZ/mini-white reporter locus. Each track shows nucleosome dyad densities for each replicate of the corresponding genotype (as described in Fig 1A). (D) Same as (C) but depicting the genomic region surrounding the integration site of the LacZ reporter transgene (dashed line). Alessandro Scacchetti et al. LSA 2018;1:e201800024 © 2018 Scacchetti et al.