Influence of K+ uptake on S. aureus metabolism.

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Influence of K+ uptake on S. aureus metabolism. Influence of K+ uptake on S. aureus metabolism. Wild-type S. aureus was inoculated to an OD600 of 0.2 in K−TB with and without 10 mM supplemental KCl at pH 6.0 (left columns), 7.3 (middle columns), and 8.6 (right columns). Following 6 h of growth, ATP generated per glucose consumed (A), acetate produced per glucose consumed (B), NAD+ and NADH levels (C), and the percentage of CTC+-stained cells (D) were determined. Results generated from the ATP, glucose, and CTC+ analyses are averages from four biological replicates (n = 4) acquired from two independent experiments with the standard deviations shown. NAD+ and NADH data are averages from three independent experiments with the standard deviations shown. Statistical analysis was performed using a one-way analysis of variance method with Tukey’s multiple-comparison test for post hoc analysis (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). Casey M. Gries et al. mSphere 2016; doi:10.1128/mSphere.00125-16