Refinement of cluster II epitopes by VHH competition ELISA

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Refinement of cluster II epitopes by VHH competition ELISA Refinement of cluster II epitopes by VHH competition ELISA. Ricin was captured onto microtiter wells coated with LE4, PH12, or TB12 and then probed with VHHs against epitope clusters I, I/II, II, or SCII (16). Refinement of cluster II epitopes by VHH competition ELISA. Ricin was captured onto microtiter wells coated with LE4, PH12, or TB12 and then probed with VHHs against epitope clusters I, I/II, II, or SCII (16). In the far-right column, ricin was captured with SylH3, an RTB-specific mAb that does not interfere with the panel of VHHs shown in the figure. The heat map is color coded from green (no competition) to red (competition). The actual OD obtained from the ELISA is presented in each box. The results shown are from one experiment of three biological replicates with essentially identical results. Greta Van Slyke et al. ImmunoHorizons 2018;2:262-273 Copyright © 2018 The Authors