20pts total 5’ 3’ Quiz 3: Biol 302 Spring2011 Name:_______________ Question 1: Draw and Label a Two Exon Complete Gene. 10pts-labeling 14 structures correctly, 8pts-labeling 10-13 structures correctly, 5pts-labeling 6-9 structures correctly. 0pts for less than 6 structures labeled correctly or for any inaccuracies. 5’ 3’ Question 2: The two differences between splicing of a transcript and gene recombination are: 1pt_______________________________ Question 3: Given the following DNA binding motif: Helix2-turn-Helix3 for a homeodomain protein. Trp48, Phe49, Asn 51 and Arg53 are invariant amongst almost all homeodomains in order to retain DNA binding. However, you want to test this idea with single amino acid substitutions. (2pts for 3 correct) What will you change Trp48 to: _____ What will you change Phe49 to: _____ What will you change Asn51 to: _____ What will you change Arg53 to: _____

Question 4: :Given the following patterns produced in Drosophila segments starting from #1(WT) Fill in appropriate segment identity in boxes below. WT #1 Minus Antp #1 minusUbx #1 plus overexpression of Antp in all segments, 2pts #1 plus overexpression of Ubx in all segments, 2pts #1 plus overexpression of AbdA in all segments, 2pts #1 T2 Antp GS - T2 Antp A1 Ubx A1 Ubx T2 Antp A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA A2 AbdA Extra Credit 2pts: The above results of segment identity can be explained in two ways Either the _____________________________________________________ or ________________________________________________________________________