Growth characteristics of strain 22A and its MDH mutant derivatives.

Slides:

Advertisements

Similar presentations

Phenotypic analysis of spo73∆ mutant.

Advertisements

Long-chain fatty acids are less abundant in the cecal contents of coconut oil-fed mice than in the cecal contents of beef tallow-fed mice. Long-chain fatty.

An inverse relationship between the centriole growth rate and period sets daughter centriole size. An inverse relationship between the centriole growth.

Long-chain fatty acids are less abundant in the cecal contents of coconut oil-fed mice than in the cecal contents of beef tallow-fed mice. Long-chain fatty.

In vitro and in vivo replication of MKWV and interferon-antagonism activity of MKWV NSs. (a) MKWV isolate MKW73 was used to inoculate cells derived from.

Levels of cytokines and chemokines in TPL-treated (from ED2) and control (H2O) mice at midgestation (ED10). Levels of cytokines and chemokines in TPL-treated.

Role of monocytes in NK cell activity.

Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ.

H-NS2 is upregulated in an hns mutant derivative of the strain E

Volume 10, Issue 3, Pages (March 2018)

Dibutyryl cAMP does not rescue staurosporine-induced filamentation of a cyr1Δ/cyr1Δ mutant. Dibutyryl cAMP does not rescue staurosporine-induced filamentation.

Influence of K+ uptake on S. aureus metabolism.

Distributions of flagellar length in long-flagella mutants before and after F-L synchronization. Distributions of flagellar length in long-flagella mutants.

Conditions that countered intracellular alkalization increased M

H-NS2-FLAG expression is upregulated in DMEM and M9 minimal medium and when cells enter the stationary growth phase. H-NS2-FLAG expression is upregulated.

Expression response of AT-rich genes to lanthanides.

Staurosporine-induced filamentation requires Cyr1 and PKA

ERMES impacts on the exposure of pathogen-associated molecular patterns during hyphal growth. ERMES impacts on the exposure of pathogen-associated molecular.

MMV is a novel antifungal agent.

Individual genes exhibit distinct stage-specific expression among female gametocytes. Individual genes exhibit distinct stage-specific expression among.

Quantification of drug cross-resistance between mutants selected for I-BET151 resistance and F0002 resistance. Quantification of drug cross-resistance.

Growth of L. monocytogenes strains AL4E, AT3E, and 10403S, derivative cured strain AT3Epc, and conjugation strains 10403SpclpL and 10403SpPL2 displayed.

Individual genes exhibit distinct female-enriched expression patterns.

Adapted viruses replicate in mismatched miR-122-expressing cells.

Doubling times and minicell production by ΔminC E

Effect of small TbTim knockdown on cell growth.

C. jejuni flaC mutants induce increased cell activation in human and chicken cells. C. jejuni flaC mutants induce increased cell activation in human and.

Binding competition between B2′B3 and B3 fragments of TcdB.

Influence of PDR1 hyperactivity and EPA1 on the colonization of the bladder and kidney by C. glabrata in the murine urinary tract infection (UTI) model.

Side-view and apical-view projections of mutant and validation strain biofilms. Side-view and apical-view projections of mutant and validation strain biofilms.

Characteristics of the Camphor-Off switch.

Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant with Triton X-100. Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant.

Antibiofilm effects of zinc pyrithione and silver sulfadiazine individually and in combination against S. aureus, A. baumannii, and P. aeruginosa. Antibiofilm.

S. pneumoniae GapA is sensitive to oxygen and H2O2 exposure.

RNA-Seq analysis of CYR1 cells in the adaptation to acid pH.

Antibody reactivity against HA1 proteins after vaccination.

Adenosine-induced osteogenic differentiation of hiPSCs involves A2bR

C. difficile induces tnaA overexpression in enterotoxigenic E. coli.

Cell density and secreted enzymes contribute to aggregate invasive growth. Cell density and secreted enzymes contribute to aggregate invasive growth. (A)

Growth, adhesion, biofilm formation, and aggregation of WT R2866 and the R2866ΔampG gene deletion mutant. Growth, adhesion, biofilm formation, and aggregation.

Arginine-to-lysine mutations conferring TRIM22 restriction and lysine-to-arginine mutations causing loss of TRIM22 restriction. Arginine-to-lysine mutations.

E. coli CFT073 and E. coli MG1655 persistence.

Proteus mirabilis strain HI4320 (WT) and isogenic mutants for dsdA, dsdC, or dsdX were cultured in LB broth overnight and diluted 1:100 into fresh LB (A),

Expression of all the GAP genes is under control of the Csy1 sensor and regulated by nitrogen sources. Expression of all the GAP genes is under control.

Susceptibility to hydrogen peroxide.

SusG LES is required for efficient packing into OMVs

IL-27 induces expression of multiple IR by CD8+ T cells.

SAM transport by Gap4 in S. cerevisiae cells.

Uptake of amino acids by C. albicans.

Using CRISPRi to knock down acm2 expression in L. plantarum WCFS1.

F-L synchronization narrows the flagellar length distribution compared to other synchronization methods. F-L synchronization narrows the flagellar length.

Wolbachia bacterial infection does not affect the expression of Imd pathway components and ROS-producing oxidases. Wolbachia bacterial infection does not.

Fig. 2. Effect of polyP3 on biofilm formation by P. gulae, P

OMVs displaying WT SusG can rescue a strain with a ΔsusG growth phenotype on starch as the carbon source. OMVs displaying WT SusG can rescue a strain with.

Expression of ylxR-containing operon revealed by lacZ and gfp fusion analysis. Expression of ylxR-containing operon revealed by lacZ and gfp fusion analysis.

Mammalian cell cytotoxicity shown by macrophage response to DM262.

A, The ratio of currents elicited by an EC50 concentration of ACh and 100 µm menthol over the current elicited by ACh alone. A, The ratio of currents elicited.

Low pH represses growth of cells with the hyphal morphology and promotes growth as pseudohyphae. Low pH represses growth of cells with the hyphal morphology.

Neutralization of CSF1 and Ad5-HRG treatment.

Mig1 influences the TOR and cell wall remodeling pathways.

DNase and proteinase K treatment of WT R2866 and R2866ΔampG gene deletion mutant biofilms. DNase and proteinase K treatment of WT R2866 and R2866ΔampG.

Dose-dependent growth inhibition assays of fluconazole and DM262.

Expression and functional characterization of Cu transporters CtrA2 and CtrC in A. nidulans. Expression and functional characterization of Cu transporters.

Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia. Growth with N-acetylglucosamine rapidly raises the.

NRG2 Affects Nitrate Accumulation and Uptake

Analysis of dCas9 expression.

GBM 112 and GBM 144 cells infected in vitro with HCMV resist growth inhibition by temozolomide. GBM 112 and GBM 144 cells infected in vitro with HCMV resist.

Mixed head-to-head competitions.

Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen. Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen.

Kros of Single grf Knockout Mutants.

Presentation transcript:

Growth characteristics of strain 22A and its MDH mutant derivatives. Growth characteristics of strain 22A and its MDH mutant derivatives. (A) Growth of strain 22A on 0.5% methanol or succinate in the presence of 30 µM La3+, Ho3+, and Lu3+. Cell yield is shown as OD600 values at 120 h. (B) Growth of strain 22A on methanol at different concentrations in the absence (open symbols) or presence (gray symbols) of 30 µM La3+. Cell yield is shown as OD600 values at 144 h. Statistical analysis was done using two-way analysis of variance with Bonferroni’s post hoc test. Only a P value of <0.05 for the effect of La3+ addition is shown. (C) Growth of the strain 22A wild type and mxaF, mxaF xoxF1, and xoxF1 mutants on 0.5% methanol in the absence (open symbols) or presence (gray symbols) of 30 µM La3+. Cell yield is shown as OD600 values at 161 h. Statistical analysis was done using two-way analysis of variance with Tukey’s post hoc tests. Only P values of interest are shown. ns, not significant statistically (P > 0.05) between the wild type and the mxaF mutant in the presence of La3+. (D) Growth of the strain 22A wild type (open symbols) and mxaF mutant (gray symbols) on 0.5% methanol in the presence of different concentrations of La3+. Cell yield is shown as OD600 values at 209 h. All experiments were performed in triplicate, and error bars show the standard deviation (SD). The specific growth rate and cell yield are shown as bars and circles, respectively. Sachiko Masuda et al. mSphere 2018; doi:10.1128/mSphere.00462-17