Glucocorticoids reduce ischemia-reperfusion-induced myocardial apoptosis in immature hearts Jeffrey M Pearl, MD, David P Nelson, MD, PhD, Steven M Schwartz,

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Presentation transcript:

Glucocorticoids reduce ischemia-reperfusion-induced myocardial apoptosis in immature hearts Jeffrey M Pearl, MD, David P Nelson, MD, PhD, Steven M Schwartz, MD, Connie J Wagner, BS, Steven M Bauer, MS, Elizabeth A Setser, BS, Jodie Y Duffy, PhD The Annals of Thoracic Surgery Volume 74, Issue 3, Pages 830-837 (September 2002) DOI: 10.1016/S0003-4975(02)03843-2

Fig 1 Left ventricular (LV) function after cardiopulmonary bypass and deep hypothermic circulatory arrest in neonatal piglets. (Top) Left ventricle systolic function (+dP/dt); (bottom) left ventricle diastolic function (−dP/dt) in neonatal piglets undergoing cardiopulmonary bypass. ∗p < 0.05, time compared with baseline. (GC = glucocorticoids; Intraop = intraoperative; Preop = preoperative.) The Annals of Thoracic Surgery 2002 74, 830-837DOI: (10.1016/S0003-4975(02)03843-2)

Fig 2 Systemic vascular resistance after cardiopulmonary bypass and deep hypothermic circulatory arrest in neonatal piglets. (GC = glucocorticoids; Intraop = intraoperative; Preop = preoperative.) The Annals of Thoracic Surgery 2002 74, 830-837DOI: (10.1016/S0003-4975(02)03843-2)

Fig 3 Ventricular intercellular adhesion molecule-1 (ICAM-1) mRNA expression. ∗p < 0.05, time compared with No GC group. (GC = glucocorticoids; Intraop = intraoperative; Preop = preoperative; LV = left ventricle; RV = right ventricle; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.) The Annals of Thoracic Surgery 2002 74, 830-837DOI: (10.1016/S0003-4975(02)03843-2)

Fig 4 Ventricular monocyte chemoattractant protein-1 (MCP-1) mRNA expression. ∗p < 0.05, time compared with No GC group. (GC = glucocorticoids; Intraop = intraoperative; Preop = preoperative; LV = left ventricle; RV = right ventricle; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.) The Annals of Thoracic Surgery 2002 74, 830-837DOI: (10.1016/S0003-4975(02)03843-2)

Fig 5 Glutathione peroxidase activity in myocardium from piglets undergoing cardiopulmonary bypass and deep hypothermic circulatory arrest. ∗p < 0.05, time compared with baseline. (GC = glucocorticoids; Intraop = intraoperative; Preop = preoperative; LV = left ventricle; RV = right ventricle.) The Annals of Thoracic Surgery 2002 74, 830-837DOI: (10.1016/S0003-4975(02)03843-2)

Fig 6 Apoptotic cells in left and right ventricular tissue of piglets measured by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay. ∗p <0.05, time compared with baseline. (GC = glucocorticoids; Intraop = intraoperative; Preop = preoperative; LV = left ventricle; RV = right ventricle.) The Annals of Thoracic Surgery 2002 74, 830-837DOI: (10.1016/S0003-4975(02)03843-2)

Fig 7 Caspase-3 activity in ventricles of neonatal piglets 120 minutes after undergoing cardiopulmonary bypass and deep hypothermic circulatory arrest. ∗p < 0.05, time compared with baseline. (GC = glucocorticoids; Intraop = intraoperative; Preop = preoperative; LV = left ventricle; RV = right ventricle.). The Annals of Thoracic Surgery 2002 74, 830-837DOI: (10.1016/S0003-4975(02)03843-2)

Fig 8 Immunoblot analyses of active caspase-3 levels in ventricles of neonatal piglets 120 minutes after undergoing cardiopulmonary bypass and deep hypothermic circulatory arrest. (Top) caspase-3 to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein ratio; (bottom) representative immunoblot in left ventricular (LV) tissue collected at 120 minutes after cardiopulmonary bypass and deep hypothermic circulatory arrest (CPB-DHCA) with antibody recognizing the 17-kDa active caspase-3 fragment of pro-caspase-3. ∗p < 0.05, time compared with no GC group. (GC = glucocorticoids; Intraop = intraoperative; Preop = preoperative; RV = right ventricle.) The Annals of Thoracic Surgery 2002 74, 830-837DOI: (10.1016/S0003-4975(02)03843-2)