Eosinophil peroxidase stimulates the release of granulocyte-macrophage colony- stimulating factor from bronchial epithelial cells Shinji Motojima, MDa,

Slides:

Advertisements

Similar presentations

W.Travis Cain, MDa, Greg Cable, PhDb, John J. Oppenheimer, MDc

Advertisements

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.

Shin-Ichi Nishikawa, MD, PhD

Immunopathogenesis of human gastrointestinal infection by Anisakis simplex Victoria del Pozo, PhDa, Ignacio Arrieta, MDa, Teresa Tuñon, MD, PhDb, Isabel.

Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.

Glucocorticoids inhibit chemokine generation by human eosinophils

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Roles of JAK kinases in human GM-CSF receptor signal transduction

Production of IL-5 and granulocyte-macrophage colony-stimulating factor by naive human mast cells activated by high-affinity IgE receptor ligation Robert.

The environmental pollutant pyrene induces the production of IL-4

Nerve growth factor is preformed in and activates human peripheral blood eosinophils Abraham Solomon, MDa,b, Luigi Aloe, PhDc, Jacob Pe’er, MDa, Joseph.

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor Akihiko Muto, PhDa, Sumiko.

Christine M. Braun, BA, Shau-Ku Huang, PhD, Gregory G

Factors affecting the cytokine production of human T cells stimulated by different modes of activation Yoshio Harada, MDa, Sumiko Watanabe, PhDa, Hans.

Effect of interleukin-5 and granulocyte-macrophage colony stimulating factor on in vitro eosinophil function: Comparison with airway eosinophils Julie.

Eosinophil apoptosis caused by theophylline, glucocorticoids, and macrolides after stimulation with IL-5 Tetsuya Adachi, MDa, Shinji Motojima, MDb, Akira.

Production of chemokines and proinflammatory and antiinflammatory cytokines by human alveolar macrophages activated by IgE receptors Philippe Gosset,

Cyclosporin H is a potent and selective competitive antagonist of human basophil activation by N-formyl-methionyl-leucyl-phenylalanine Amato de Paulis,

Estrogen enhances immunoglobulin production by human PBMCs

Prostaglandin E2 control of T cell cytokine production is functionally related to the reduced lymphocyte proliferation in atopic dermatitis Sai Chan,

Deficient prostaglandin E2 production by bronchial fibroblasts of asthmatic patients, with special reference to aspirin-induced asthma Malgorzata Pierzchalska,

Granulocyte-macrophage colony-stimulating factor and IL-5 activate mitogen-activated protein kinase through Jak2 kinase and phosphatidylinositol 3-kinase.

The histamine-cytokine network in allergic inflammation

Pathophysiology of severe asthma

Conversion of the CD4+ T cell profile from TH2-dominant type to TH1-dominant type after varicella-zoster virus infection in atopic dermatitis Takao Fujimura,

Basophils from patients with allergic asthma show a primed phenotype

Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A Satoshi Matsukura, MD, Fumio Kokubu,

Cristiana Stellato, MD, PhDa, Jun Atsuta, MD, PhDb, Carol A

Glucocorticoids inhibit IL-4 and mitogen-induced IL-4Rα chain expression by different posttranscriptional mechanisms Lourdes Mozo, PhDa, Abel Gayo, BSa,

Chronologic analysis of in situ cytokine expression in mite allergen-induced dermatitis in atopic subjects Nobuo Yamada, MDa, Motoshi Wakugawa, MDa,

Yanli Ouyang, MD, PhDa, Nisha Virasch, BSa, Ping Hao, MDa, Michael T

Exogenous IFN-β has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus Julie.

Pathophysiology of the inflammatory response

Glucocorticoids inhibit chemokine generation by human eosinophils

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Delayed eosinophil programmed cell death in vitro: A common feature of inhalant allergy and extrinsic and intrinsic atopic dermatitis Bettina Wedi, MD,

Chemokines induce eosinophil degranulation through CCR-3

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor Akihiko Muto, PhDa, Sumiko.

Epithelial cells as regulators of airway inflammation

Effect of ozone and nitrogen dioxide on the release of proinflammatory mediators from bronchial epithelial cells of nonatopic nonasthmatic subjects and.

Signals and nuclear factors that regulate the expression of interleukin-4 and interleukin- 5 genes in helper T cells Hyun Jun Lee, MSa, Ikuo Matsuda,

Specific versus cooperative regulatory mechanisms of the cytokine genes that are clustered on the same chromosome Naoko Koyano-Nakagawa, PhD, Ken-ichi.

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Ganglioside GQ1b enhances Ig production by human PBMCs

The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma James L. Lordan,

News & Notes Journal of Allergy and Clinical Immunology

IL-9 expression by human eosinophils: Regulation by IL-1β and TNF-α

Human granulocyte-macrophage colony-stimulating factor (hGM-CSF)–dependent in vitro and in vivo proliferation and differentiation of all hematopoietic.

Keratinocytes Inhibit Expression of Connective Tissue Growth Factor in Fibroblasts In Vitro by an Interleukin-1α-Dependent Mechanism Daniel Nowinski,

Effect of rush immunotherapy on airway inflammation and airway hyperresponsiveness after bronchoprovocation with allergen in asthma Yasurou Kohno, MDa,

P38 Mitogen-activated protein kinase–induced glucocorticoid receptor phosphorylation reduces its activity: Role in steroid-insensitive asthma Elvis Irusen,

Expression and function of histamine receptors in human monocyte-derived dendritic cells Marco Idzko, MDa, Andrea la Sala, PhDb, Davide Ferrari, PhDc,

Blood eosinophils from atopic donors express messenger RNA for the α, β, and γ subunits of the high-affinity IgE receptor (FcϵRI) and intracellular, but.

Larry L. Thomas, PhD, Michelle D. Haskell, Emmanuel U

A thiol antioxidant regulates IgE isotype switching by inhibiting activation of nuclear factor-κB Yukiyoshi Yanagihara, PhD, Yuji Basaki, MSc, Keiichi.

Diesel exhaust particles stimulate human airway epithelial cells to produce cytokines relevant to airway inflammation in vitro Takayuki Ohtoshi, MDa,

Pia J. Hauk, MDa, Qutayba A. Hamid, MD, PhDc, George P

Nedocromil sodium and airway inflammation in vivo and in vitro

Abnormalities of cell and mediator levels in bronchoalveolar lavage fluid of patients with mild asthma Sally E. Wenzel, MD Journal of Allergy and Clinical.

IL-4 upregulates FcϵRI α-chain messenger RNA in eosinophils

TH2 Cytokine-enhanced and TGF-β-enhanced vascular endothelial growth factor production by cultured human airway smooth muscle cells is attenuated by IFN-γ.

Mechanisms of inhibition of IgE synthesis by nedocromil sodium: Nedocromil sodium inhibits deletional switch recombination in human B cells Richard K.S.

Comparison of ciliary activity and inflammatory mediator release from bronchial epithelial cells of nonatopic nonasthmatic subjects and atopic asthmatic.

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.

Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring.

News & Notes Journal of Allergy and Clinical Immunology

Diisocyanate antigen–enhanced production of monocyte chemoattractant protein-1, IL- 8, and tumor necrosis factor-α by peripheral mononuclear cells of workers.

Myosin light chain kinase mediates eosinophil chemotaxis in a mitogen-activated protein kinase–dependent manner Tetsuya Adachi, MDa, Susan Stafford,

Effect of fexofenadine on eosinophil-induced changes in epithelial permeability and cytokine release from nasal epithelial cells of patients with seasonal.

Presentation transcript:

Eosinophil peroxidase stimulates the release of granulocyte-macrophage colony- stimulating factor from bronchial epithelial cells Shinji Motojima, MDa, Tetsuya Adachi, MDa, Ken-ichi Manaka, PhDb, Masafumi Arima, MDa, Takeshi Fukuda, MDa, Sohei Makino, MDa Journal of Allergy and Clinical Immunology Volume 98, Issue 6, Pages S216-S223 (December 1996) DOI: 10.1016/S0091-6749(96)70069-6 Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 1 Effect of EPO on release of GM-CSF from BEAS-2B cells. BEAS-2B cells were first washed with phosphate-buffered saline. EPO was then applied to BEAS-2B cells and incubated for 15 minutes at 37° C. Cells were washed twice with HD-F12 medium and were again incubated with 1 ml of HD-F12 at 37° C. Supernatants were harvested at 3, 6, or 24 hours, and concentration of GM-CSF was measured. Journal of Allergy and Clinical Immunology 1996 98, S216-S223DOI: (10.1016/S0091-6749(96)70069-6) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 2 Effect of various substances on release of GM-CSF from BEAS-2B cells in 24-hour culture supernatants. BEAS-2B cells were exposed to EPO, poly-L-arginine, poly-L-lysine, and poly-L-glutamic acid for 15 minutes and washed before incubation with 1 ml of HD-F12. Human recombinant IL-1β and catalase were cocultured with cells for entire 24-hour incubation period. Cells were exposed to CHX for 30 minutes before exposure to EPO and then were cultured in its presence for 24 hours. Heparin was mixed with EPO before addition to wells with BEAS-2B cells. Journal of Allergy and Clinical Immunology 1996 98, S216-S223DOI: (10.1016/S0091-6749(96)70069-6) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Effect of EPO + H2O2 + halide system on release of GM-CSF from BEAS-2B cells in 24-hour culture supernatants. BEAS-2B cells were first washed with phosphate-buffered saline. EPO was then applied to BEAS-2B cells and incubated for 15 minutes at 37° C. Cells were washed twice with HD-F12 medium and were again incubated with 1 ml of HD-F12 at 37° C in presence of 10-5 mol/L H2O2 and 10-4 mol/L NaBr. To compare with EPO system, individual components included in EPO system were added separately. Journal of Allergy and Clinical Immunology 1996 98, S216-S223DOI: (10.1016/S0091-6749(96)70069-6) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Expression of mRNA for GM-CSF and β-actin detected by Northern blotting. RNA extracted from BEAS-2B cells pretreated with 5.9 × 10-7 mol/L or those incubated with medium alone (control) were subjected to Northern blotting. Journal of Allergy and Clinical Immunology 1996 98, S216-S223DOI: (10.1016/S0091-6749(96)70069-6) Copyright © 1996 Mosby, Inc. Terms and Conditions