Regina Célia Teixeira Gomes, M. Sc. , Carina Verna, M. Sc

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Concentration and distribution of hyaluronic acid in mouse uterus throughout the estrous cycle Regina Célia Teixeira Gomes, M.Sc., Carina Verna, M.Sc., Helena Bonciani Nader, M.Sc., Ph.D., Ricardo dos Santos Simões, M.D., Juliana L. Dreyfuss, Ph.D., João Roberto Maciel Martins, M.D., Ph.D., Edmund C. Baracat, M.D., Ph.D., Manuel de Jesus Simões, M.D., Ph.D., José Maria Soares, M.D., Ph.D Fertility and Sterility Volume 92, Issue 2, Pages 785-792 (August 2009) DOI: 10.1016/j.fertnstert.2008.07.005 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Standard curve obtained by fluorescence-based assay for hyaluronan. Each individual point is the mean of three determinations. The vertical bars show the SDs of the measurements. Fertility and Sterility 2009 92, 785-792DOI: (10.1016/j.fertnstert.2008.07.005) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 HA concentrations in the uterine horns of regularly cycling adult mice. Bars represent mean ± SD. Analysis of variance P-values for comparison of phases: ∗P<.05. Fertility and Sterility 2009 92, 785-792DOI: (10.1016/j.fertnstert.2008.07.005) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 HA immunohistochemical detection on transversal sections of the intermediate third of the mouse horns during the different phases of the estrous cycle. Notice the intense brownish staining for HA, indicating high HA concentration throughout the endometrium (E) and within the myometrial layers (M). A = negative control. Bar for all panels = 50 μm. Fertility and Sterility 2009 92, 785-792DOI: (10.1016/j.fertnstert.2008.07.005) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 4 HA receptor immunohistochemical detection on transversal sections of the intermediate third of the mouse horns during the different phases of the estrous cycle. Notice the intense brownish staining for HA, indicating high HA concentration throughout the endometrium (E) and within the myometrial layers (M). B = negative control. Bar for all panels = 50 μm. Fertility and Sterility 2009 92, 785-792DOI: (10.1016/j.fertnstert.2008.07.005) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions