SDS-PAGE analysis of mature seed from lines Patriot and PI

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SDS-PAGE analysis of mature seed from lines Patriot and PI 603408. SDS-PAGE analysis of mature seed from lines Patriot and PI 603408. (A) SDS-PAGE analysis of total seed proteins (samples 1 and 2) or calcium carbonate enrichment of seed storage proteins (samples 3 and 4), and (B) immunoblot analysis of total seed proteins (samples 1 and 2) or calcium carbonate enrichment of seed storage proteins (samples 3 and 4) with anti-glycinin 4/5 antibodies. Samples 1 and 3 are from seed of cultivar Patriot and samples 2 and 4 are from seed of PI 603408. Arrow indicates protein band polymorphism between genotypes. SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis. Jason D. Gillman et al. G3 2017;7:2345-2352 ©2017 by Genetics Society of America