Alterations in aortic endothelial cell morphology and cytoskeletal protein synthesis during cyclic tensional deformation Bauer E. Sumpio, M.D., Ph.D.,

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Presentation transcript:

Alterations in aortic endothelial cell morphology and cytoskeletal protein synthesis during cyclic tensional deformation Bauer E. Sumpio, M.D., Ph.D., Albert J. Banes, Ph.D., Michael Buckley, D.D.S., George Johnson, M.D. Journal of Vascular Surgery Volume 7, Issue 1, Pages 130-138 (January 1988) DOI: 10.1016/0741-5214(88)90386-2 Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Rhodamine phalloidin staining of F-actin in bovine aortic endothelial cells after 5 days of static (A) or cyclic (B) tension. The cells grown under static conditions displayed a more diffuse distribution of actin. The cells subjected to tension had a more polygonal shape, pseudopods, actin stress fibers, and peripheral vacuoles. (Magnification × 1000.) Journal of Vascular Surgery 1988 7, 130-138DOI: (10.1016/0741-5214(88)90386-2) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Photograph of aortic endothelial cells on the periphery of culture plate subjected to 10 seconds of 24% elongation and 10 seconds of relaxation for 5 days and then stained with crystal violet. The cells were randomly oriented. (Magnification × 40.) Journal of Vascular Surgery 1988 7, 130-138DOI: (10.1016/0741-5214(88)90386-2) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Two-dimensional gel analysis of aortic bovine endothelial cells after 5 days of static tension. Endothelial cells were incubated overnight with 35S-methionine, and the cells were harvested as described in the Material and Methods section. The samples (specific activity. 28,414 dpm/μg) were loaded at 300,000 dpm/gel onto a broad-range (pH 3 to 10, acid end to the left) ampholine, first dimension, and 12.5% acrylamide, second dimension. Autoradiographs were prepared from two-dimensional slab gels. Journal of Vascular Surgery 1988 7, 130-138DOI: (10.1016/0741-5214(88)90386-2) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Two-dimensional gel analysis of aortic bovine endothelial cells after 5 days of cyclic tension. Endothelial cells were incubated overnight with 35S-methionine, and the cells were harvested as described in the Material and Methods section. The samples (specific activity. 44,336 dpm/μg) were loaded at 300,000 dpm/gel onto a broad-range (pH 3 to 10, acid end to the left) ampholine, first dimension, and 12.5% acrylamide, second dimension. Autoradiographs were prepared from two-dimensional slab gels. Arrows point to some protein spots that were increased by cyclic stretch. Journal of Vascular Surgery 1988 7, 130-138DOI: (10.1016/0741-5214(88)90386-2) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Comparison of protein spots on two-dimensional gels of static (control) and stretched aortic endothelial cells by a correlation plot. For each protein of the correlated samples, increases and decreases in its relative disintegration-per-minute value are plotted on the x axis for the control experiment and its increase or decrease in relative disintegrations per minute for the stretch experiment are plotted on the y axis. N = number of spots compared; r = correlation coefficient. Journal of Vascular Surgery 1988 7, 130-138DOI: (10.1016/0741-5214(88)90386-2) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions