Disseminated Mycobacterium chimaera infection associated with heater–cooler units after aortic valve surgery without endocarditis  Tannaz Asadi, MD, Katherine.

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Disseminated Mycobacterium chimaera infection associated with heater–cooler units after aortic valve surgery without endocarditis  Tannaz Asadi, MD, Katherine Mullin, MD, Eric Roselli, MD, Douglas Johnston, MD, Carmela D. Tan, MD, E. Rene Rodriguez, MD, Steven Gordon, MD  The Journal of Thoracic and Cardiovascular Surgery  Volume 155, Issue 6, Pages 2369-2374 (June 2018) DOI: 10.1016/j.jtcvs.2017.12.049 Copyright © 2017 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Gross examination of the periaortic debris shows yellow-to-tan tissue with some fibrous tissue strands (white tissue). The Journal of Thoracic and Cardiovascular Surgery 2018 155, 2369-2374DOI: (10.1016/j.jtcvs.2017.12.049) Copyright © 2017 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 A, Light micrograph of the perigraft tissue shows abundant granulation tissue, rich in polymorphonuclear leukocytes undergoing karyorrhexis and conspicuous macrophages with foamy cytoplasm. Rare multinucleated giant cells were also present (inset). ×200, hematoxylin and eosin. B, The same microscopic field shows no distinct mycobacteria at low magnification but many areas of foamy macrophages. ×200, Fite–Faraco stain. C, Multiple mycobacterial organisms are seen within foamy macrophages on oil-immersion microscopy. The inset shows a “banding” pattern of stain of the mycobacteria. ×1000, Fite–Faraco stain. The Journal of Thoracic and Cardiovascular Surgery 2018 155, 2369-2374DOI: (10.1016/j.jtcvs.2017.12.049) Copyright © 2017 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 A, Similar to the first case, these images show abscess formation with karyorrhectic polymorphonuclear leukocytes at an interface with foamy macrophage inflammatory infiltrate. ×600, hematoxylin and eosin. B, Greater magnification shows a mycobacterium (arrow) inside a macrophage. ×1000, Fite–Faraco stain. The Journal of Thoracic and Cardiovascular Surgery 2018 155, 2369-2374DOI: (10.1016/j.jtcvs.2017.12.049) Copyright © 2017 The American Association for Thoracic Surgery Terms and Conditions

Gross examination of the periaortic debris shows an organizing abscess material. The Journal of Thoracic and Cardiovascular Surgery 2018 155, 2369-2374DOI: (10.1016/j.jtcvs.2017.12.049) Copyright © 2017 The American Association for Thoracic Surgery Terms and Conditions

Video 1 The challenges of disseminated M chimaera infection associated with heater–cooler units after valve-replacement surgery. The Journal of Thoracic and Cardiovascular Surgery 2018 155, 2369-2374DOI: (10.1016/j.jtcvs.2017.12.049) Copyright © 2017 The American Association for Thoracic Surgery Terms and Conditions