Development of a Diagnostic Microarray Assay to Assess the Risk of Recurrence of Prostate Cancer Based on PITX2 DNA Methylation Philipp Schatz, Dimo.

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Presentation transcript:

Development of a Diagnostic Microarray Assay to Assess the Risk of Recurrence of Prostate Cancer Based on PITX2 DNA Methylation Philipp Schatz, Dimo Dietrich, Thomas Koenig, Matthias Burger, Antje Lukas, Ina Fuhrmann, Glen Kristiansen, Robert Stoehr, Matthias Schuster, Ralf Lesche, Gunter Weiss, John Corman, Arndt Hartmann The Journal of Molecular Diagnostics Volume 12, Issue 3, Pages 345-353 (May 2010) DOI: 10.2353/jmoldx.2010.090088 Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Twenty-one methylation detection probes and 43 probe pairings of the Epichip PITX2. Probes specific for the methylated state (rows) and specific for the unmethylated state (columns) of PITX2 are displayed. Numbered (I-XLIII) cells of the matrix indicate 43 probe pairings used for methylation signal extraction. Capital letters used in the sequences indicate CpG context. The Journal of Molecular Diagnostics 2010 12, 345-353DOI: (10.2353/jmoldx.2010.090088) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Kaplan Meier Analysis of the probability of remaining free of biochemical relapse after prostatectomy treatment in a cohort of 157 patients. Solid lines correspond to classification by the microarray Epichip PITX2 assay, and dashed lines correspond to comparative method real-time PCR (QM) assay. The Journal of Molecular Diagnostics 2010 12, 345-353DOI: (10.2353/jmoldx.2010.090088) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Scatter plot of numerical methylation values for a cohort of 157 patients: Epichip PITX2 (x axis) and comparative method QM real-time PCR (y axis). Solid lines represent the decision point (cut-off) of the respective assay. Dashed lines define border zone limits for the Epichip PITX2. The Journal of Molecular Diagnostics 2010 12, 345-353DOI: (10.2353/jmoldx.2010.090088) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Visualized results from the cut-off transfer (16 patient samples that were known to be close to the decision point [cut-off] were measured [1-3 valid measurements]), the daily use reproducibility (13 valid LTS and HTS measurements), and the repeatability (40 valid HTS measurements). The results are shown in comparison with the calibrated methylation scores of the 157 prostatectomy samples. The clinical decision point and the resulting borderline are depicted. The Journal of Molecular Diagnostics 2010 12, 345-353DOI: (10.2353/jmoldx.2010.090088) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

The Journal of Molecular Diagnostics 2010 12, 345-353DOI: (10 The Journal of Molecular Diagnostics 2010 12, 345-353DOI: (10.2353/jmoldx.2010.090088) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions