S. Hendriks, E. A. F. Dancet, A. Meissner, F. van der Veen, M. H

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Presentation transcript:

Perspectives of infertile men on future stem cell treatments for nonobstructive azoospermia S. Hendriks, E.A.F. Dancet, A. Meissner, F. van der Veen, M.H. Mochtar, S. Repping Reproductive BioMedicine Online Volume 28, Issue 5, Pages 650-657 (May 2014) DOI: 10.1016/j.rbmo.2014.01.011 Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 The making of artificial sperm followed by ICSI: a somatic cell (in this case a fibroblast) is transformed into a stem cell, which is then differentiated into mature sperm cells in vitro. The female partner receives hormone treatment, after which mature oocytes are retrieved from the ovary. The previously generated sperm cells are then used to fertilize the oocyte in vitro. After successful fertilization, the embryo is placed into the uterus to achieve pregnancy. Reproductive BioMedicine Online 2014 28, 650-657DOI: (10.1016/j.rbmo.2014.01.011) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Autotransplantation of in vitro proliferated spermatogonial stem cells (SSCs): SSCs are removed from the testes by means of a testicular biopsy, these SSCs are then proliferated in vitro and subsequently transplanted back into the testes. As a result, sperm production in the testes of this patient can increase so that mature spermatozoa can appear in the ejaculate, allowing natural conception. Reproductive BioMedicine Online 2014 28, 650-657DOI: (10.1016/j.rbmo.2014.01.011) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions